Explorations into Peptide Nucleic Acid Contrast Agents as Emerging Scaffolds for Breakthrough Solutions in Medical Imaging and Diagnosis

Rudiger M. Exner; Stephen Paisey; James Redman; Sofia Pascu

doi:10.1021/acsomega.1c03994

Explorations into Peptide Nucleic Acid Contrast Agents as Emerging Scaffolds for Breakthrough Solutions in Medical Imaging and Diagnosis

Rudiger M. Exner, Stephen Paisey, James Redman, Sofia Pascu

Cardiff University

Research output: Contribution to journal › Article › peer-review

4 Citations (SciVal)

Abstract

Peptide nucleic acids (PNAs, nucleic acid analogues with a peptide backbone rather than a phosphoribosyl backbone) have emerged as promising chemical agents in antigene or antisense therapeutics, as splicing modulators or in gene editing. Their main benefits, compared to DNA or RNA agents, are their biochemical stability and the lack of negative charges throughout the backbone, leading to negligible electrostatic interaction with the strand with which they are hybridizing. As a result, hybridization of PNA strands with DNA or RNA strands leads to higher binding energies and melting temperatures. A lack of natural transporters, however, necessitates the formation of PNA-containing chimeras or the formulation of nanoparticular cell delivery methods. Here, we set out to explore the progress made in using imaging agents based on PNAs in diagnostic applications and highlight selected developments and challenges.

Original language	English
Journal	ACS OMEGA
DOIs	https://doi.org/10.1021/acsomega.1c03994
Publication status	Published - 19 Oct 2021

Funding

S.I.P. acknowledges funding from ERC Consolidator Grant O2Sense 617107 (2014–2020) and ERC PoC grant Tools-To-Sense (963937), EPSRC (EP/K017160/1 “New manufacturable approaches to the deposition and patterning of graphene materials”), Innovate UK (previously Technology Strategy Board - CR&D, TS/K001035/1), University of Bath (UoB) Impact fund, EPSRC Centre for Doctoral Training Centre for Sustainable Chemical Technologies (EP/G03768X/1). We thank the EPSRC for funding through the Healthcare theme of the CSCT at Bath, GW4 PET and Cancer Research at Bath (CR@B) network for this collaboration opportunity, University of Bath for a URSA studentship to R.M.E., and ERC O2Sense and Tools-to-Sense grants to S.I.P. for their continuing funding support since 2014.

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abstract = "Peptide nucleic acids (PNAs, nucleic acid analogues with a peptide backbone rather than a phosphoribosyl backbone) have emerged as promising chemical agents in antigene or antisense therapeutics, as splicing modulators or in gene editing. Their main benefits, compared to DNA or RNA agents, are their biochemical stability and the lack of negative charges throughout the backbone, leading to negligible electrostatic interaction with the strand with which they are hybridizing. As a result, hybridization of PNA strands with DNA or RNA strands leads to higher binding energies and melting temperatures. A lack of natural transporters, however, necessitates the formation of PNA-containing chimeras or the formulation of nanoparticular cell delivery methods. Here, we set out to explore the progress made in using imaging agents based on PNAs in diagnostic applications and highlight selected developments and challenges.",

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AU - Paisey, Stephen

AU - Redman, James

AU - Pascu, Sofia

PY - 2021/10/19

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AB - Peptide nucleic acids (PNAs, nucleic acid analogues with a peptide backbone rather than a phosphoribosyl backbone) have emerged as promising chemical agents in antigene or antisense therapeutics, as splicing modulators or in gene editing. Their main benefits, compared to DNA or RNA agents, are their biochemical stability and the lack of negative charges throughout the backbone, leading to negligible electrostatic interaction with the strand with which they are hybridizing. As a result, hybridization of PNA strands with DNA or RNA strands leads to higher binding energies and melting temperatures. A lack of natural transporters, however, necessitates the formation of PNA-containing chimeras or the formulation of nanoparticular cell delivery methods. Here, we set out to explore the progress made in using imaging agents based on PNAs in diagnostic applications and highlight selected developments and challenges.

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Explorations into Peptide Nucleic Acid Contrast Agents as Emerging Scaffolds for Breakthrough Solutions in Medical Imaging and Diagnosis

Abstract

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