Abstract

Introduction/objectives: It is well documented that implant loosening rate in sickle cell disease patients is higher than seen in patients with THR from other indications. The Hypoxic inducible factor(HIF) - is activated in the microcellular hypoxic environment and this through a cascade of other enzymatic reactions promotes the activity of other factors and further help enhance angiogenesis and osteogenesis. The aim of this study was to investigate and propose a potential model for investigating osseointegration in a hypoxic microcellular environment using osteoblasts(MG63). Methods: Human MG63 osteoblastic cells were cultured under normoxia and hypoxic conditions (20%; and 1% oxygen saturation) for 72 hours under two different condition- with and without cobalt chloride. The samples cultured under normoxic condtions without cobalt chloride acted as control. Using qualitative polymerase chain reaction-(qPCR) - HIF expression was assessed under the above conditions in relation to the control. Results: The results showed there was significant expression of the HIF 1 alpha protein under hypoxic condition with cobalt chloride in comparison with the control samples- all at 72hours incubation. Mann-Whitney U test was used to deduce level of significance of fold change.(p=0.002; <0.05). This was deemed as there being a significant difference in the level of expression of HIF compared to the control. Conclusion: The results show that the hypoxic inducible factor can be expressed using the above tested experimental invitro-model with significant results which can be a foundation for further research into improving hip implant prosthesis design to help enhance osseointegration in sickle cell disease patient with AVN.
Original languageEnglish
Publication statusPublished - Sep 2018
Event13th Congress of the European Hip Society - The Hague, Netherlands
Duration: 20 Sep 201822 Sep 2018
http://ehs-congress.org/

Conference

Conference13th Congress of the European Hip Society
CountryNetherlands
CityThe Hague
Period20/09/1822/09/18
Internet address

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Osseointegration
Bone Diseases
Sickle Cell Anemia
Necrosis
Prosthesis Design
Hip Prosthesis
Nonparametric Statistics
Osteoblasts
Osteogenesis
Cultured Cells
Theoretical Models
Oxygen
Polymerase Chain Reaction
Research
cobaltous chloride
Proteins

Cite this

Experimental study to investigate a potential model for improved osseointegration in sickle cell bone disease patients with avascular necrosis. / George, Akintunde; Ellis, Marianne; Gill, Harinderjit.

2018. Paper presented at 13th Congress of the European Hip Society, The Hague, Netherlands.

Research output: Contribution to conferencePaper

George, A, Ellis, M & Gill, H 2018, 'Experimental study to investigate a potential model for improved osseointegration in sickle cell bone disease patients with avascular necrosis' Paper presented at 13th Congress of the European Hip Society, The Hague, Netherlands, 20/09/18 - 22/09/18, .
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abstract = "Introduction/objectives: It is well documented that implant loosening rate in sickle cell disease patients is higher than seen in patients with THR from other indications. The Hypoxic inducible factor(HIF) - is activated in the microcellular hypoxic environment and this through a cascade of other enzymatic reactions promotes the activity of other factors and further help enhance angiogenesis and osteogenesis. The aim of this study was to investigate and propose a potential model for investigating osseointegration in a hypoxic microcellular environment using osteoblasts(MG63). Methods: Human MG63 osteoblastic cells were cultured under normoxia and hypoxic conditions (20{\%}; and 1{\%} oxygen saturation) for 72 hours under two different condition- with and without cobalt chloride. The samples cultured under normoxic condtions without cobalt chloride acted as control. Using qualitative polymerase chain reaction-(qPCR) - HIF expression was assessed under the above conditions in relation to the control. Results: The results showed there was significant expression of the HIF 1 alpha protein under hypoxic condition with cobalt chloride in comparison with the control samples- all at 72hours incubation. Mann-Whitney U test was used to deduce level of significance of fold change.(p=0.002; <0.05). This was deemed as there being a significant difference in the level of expression of HIF compared to the control. Conclusion: The results show that the hypoxic inducible factor can be expressed using the above tested experimental invitro-model with significant results which can be a foundation for further research into improving hip implant prosthesis design to help enhance osseointegration in sickle cell disease patient with AVN.",
author = "Akintunde George and Marianne Ellis and Harinderjit Gill",
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note = "13th Congress of the European Hip Society ; Conference date: 20-09-2018 Through 22-09-2018",
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AU - Gill, Harinderjit

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N2 - Introduction/objectives: It is well documented that implant loosening rate in sickle cell disease patients is higher than seen in patients with THR from other indications. The Hypoxic inducible factor(HIF) - is activated in the microcellular hypoxic environment and this through a cascade of other enzymatic reactions promotes the activity of other factors and further help enhance angiogenesis and osteogenesis. The aim of this study was to investigate and propose a potential model for investigating osseointegration in a hypoxic microcellular environment using osteoblasts(MG63). Methods: Human MG63 osteoblastic cells were cultured under normoxia and hypoxic conditions (20%; and 1% oxygen saturation) for 72 hours under two different condition- with and without cobalt chloride. The samples cultured under normoxic condtions without cobalt chloride acted as control. Using qualitative polymerase chain reaction-(qPCR) - HIF expression was assessed under the above conditions in relation to the control. Results: The results showed there was significant expression of the HIF 1 alpha protein under hypoxic condition with cobalt chloride in comparison with the control samples- all at 72hours incubation. Mann-Whitney U test was used to deduce level of significance of fold change.(p=0.002; <0.05). This was deemed as there being a significant difference in the level of expression of HIF compared to the control. Conclusion: The results show that the hypoxic inducible factor can be expressed using the above tested experimental invitro-model with significant results which can be a foundation for further research into improving hip implant prosthesis design to help enhance osseointegration in sickle cell disease patient with AVN.

AB - Introduction/objectives: It is well documented that implant loosening rate in sickle cell disease patients is higher than seen in patients with THR from other indications. The Hypoxic inducible factor(HIF) - is activated in the microcellular hypoxic environment and this through a cascade of other enzymatic reactions promotes the activity of other factors and further help enhance angiogenesis and osteogenesis. The aim of this study was to investigate and propose a potential model for investigating osseointegration in a hypoxic microcellular environment using osteoblasts(MG63). Methods: Human MG63 osteoblastic cells were cultured under normoxia and hypoxic conditions (20%; and 1% oxygen saturation) for 72 hours under two different condition- with and without cobalt chloride. The samples cultured under normoxic condtions without cobalt chloride acted as control. Using qualitative polymerase chain reaction-(qPCR) - HIF expression was assessed under the above conditions in relation to the control. Results: The results showed there was significant expression of the HIF 1 alpha protein under hypoxic condition with cobalt chloride in comparison with the control samples- all at 72hours incubation. Mann-Whitney U test was used to deduce level of significance of fold change.(p=0.002; <0.05). This was deemed as there being a significant difference in the level of expression of HIF compared to the control. Conclusion: The results show that the hypoxic inducible factor can be expressed using the above tested experimental invitro-model with significant results which can be a foundation for further research into improving hip implant prosthesis design to help enhance osseointegration in sickle cell disease patient with AVN.

M3 - Paper

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