Exercise-induced amplification of mitogen-stimulated oxidative burst in whole blood is strongly influenced by neutrophil counts during and following exercise

Alice Lester, Gabrielle Vickers, Laura Macro, Alex Gudgeon, Alice Bonham-Carter, John Campbell, James Turner

Research output: Contribution to journalArticlepeer-review

Abstract

This study characterised the effect of moderate- or vigorous-intensity exercise on leukocyte counts, using fingertip sampling, and mitogen-stimulated oxidative burst, measured in whole blood with a point-of-care test. In a randomised crossover design, thirteen healthy adults (mean +/- SD age: 22 +/- 2 years; seven male, six female) cycled for 30-minutes, once at 52 +/- 5% V̇O2peak and on another occasion at 74 +/- 9% V̇O2peak. Blood was sampled at baseline, immediately post-exercise, and 15- and 60-minutes post-exercise. The leukocyte differential and mitogen-stimulated Reactive Oxygen Species (ROS) production were assessed. Lymphocytes increased immediately post-exercise and decreased below pre-exercise levels 15- and 60-minutes later. Lymphocyte mobilisation immediately post-exercise was 59 +/- 36% greater with vigorous- compared to moderate-intensity exercise (P<0.01). Neutrophils increased immediately after exercise (38 +/- 19 %, P<0.01) remaining elevated 60-minutes later (50 +/- 34%, P<0.01; averaged across intensities) and did not differ between intensities (P=0.259). Mitogen-stimulated ROS production was amplified immediately (+32 +/- 37%, P<0.01) and 60-minutes post-exercise (+56 +/- 57%, P<0.01; averaged across intensities) compared to rest and did not differ with intensity (P=0.739). Exercise-induced amplification of ROS production was abolished when correcting for neutrophil, monocyte and platelet counts and correlated most strongly with neutrophil mobilisation immediately (r = 0.709, P<0.01) and 60-minutes after vigorous exercise (r = 0.687, P<0.01). Leukocyte kinetics can be assessed using fingertip blood sampling in exercise settings. Exercise-induced amplification of oxidative burst is detectable with a point-of-care test, but results are strongly influenced by neutrophil counts, which may not be routinely quantified.
Original languageEnglish
Article numbere15010
JournalPhysiological Reports
Volume9
Issue number17
Early online date8 Sep 2021
DOIs
Publication statusE-pub ahead of print - 8 Sep 2021

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