Abstract
Fluorescent probes have become promising tools for monitoring the concentration of peroxynitrite, which is linked to many diseases. However, despite focusing on developing numerous peroxynitrite based fluorescent probes, limited emphasis is placed on their sensing mechanism. Here, we investigated the sensing mechanism of a peroxynitrite fluorescent probe, named BHID-Bpin, with a focus on the relevant excited state dynamics. The photoexcited BHID-Bpin relaxes to its ground state via an efficient nonradiative process (∼300 ps) due to the presence of a minimum energy conical intersection between its first excited state and ground state. However, upon reacting with peroxynitrite, the Bpin moiety is cleaved from BHID-Bpin and BHID is formed. The formed BHID exhibits strong dual band fluorescence which is caused by an ultrafast excited-state intramolecular proton transfer process (∼1 ps).
| Original language | English |
|---|---|
| Pages (from-to) | 13506-13514 |
| Number of pages | 9 |
| Journal | Physical Chemistry Chemical Physics |
| Volume | 26 |
| Issue number | 17 |
| Early online date | 3 Apr 2024 |
| DOIs | |
| Publication status | Published - 3 Apr 2024 |
Funding
Financial support of this work from the National Natural Science Foundation of China (No. 21803077) and the Novo Nordisk Foundation (NNF22OC0073582) is acknowledged. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. YH acknowledges the support from the China Scholarship Council (No. 202006150002).
| Funders | Funder number |
|---|---|
| University of Bath | |
| National Natural Science Foundation of China | 21803077 |
| National Natural Science Foundation of China | |
| Novo Nordisk Fonden | NNF22OC0073582 |
| Novo Nordisk Fonden | |
| Henan Normal University | 2020ZD01 |
| Henan Normal University | |
| China Scholarship Council | 202006150002 |
| China Scholarship Council |