Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems

Gavin Hazell; Thomas Arnold; Robert D. Barker; Luke A. Clifton; Nina Juliane Steinke; Cecilia Tognoloni; Karen J. Edler

doi:10.1021/acs.langmuir.6b02927

Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems

Gavin Hazell, Thomas Arnold, Robert D. Barker, Luke A. Clifton, Nina Juliane Steinke, Cecilia Tognoloni, Karen J. Edler

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

39 Citations (SciVal)

Abstract

Styrene-alt-maleic acid lipid particles (SMALPs) are self-assembled discoidal structures composed of a polymer belt and a segment of lipid bilayer, which are capable of encapsulating membrane proteins directly from the cell membrane. Here we present evidence of the exchange of lipids between such "nanodiscs" and lipid monolayers adsorbed at either solid-liquid or air-liquid interfaces. This behavior has important implications for the potential uses of nanodiscs.

Original language	English
Pages (from-to)	11845-11853
Number of pages	9
Journal	Langmuir
Volume	32
Issue number	45
DOIs	https://doi.org/10.1021/acs.langmuir.6b02927
Publication status	Published - 15 Nov 2016

Access to Document

10.1021/acs.langmuir.6b02927Licence: CC BY-NC-ND

Cite this

@article{724ba801b5354c249c1d10dcb3004fda,

title = "Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems",

abstract = "Styrene-alt-maleic acid lipid particles (SMALPs) are self-assembled discoidal structures composed of a polymer belt and a segment of lipid bilayer, which are capable of encapsulating membrane proteins directly from the cell membrane. Here we present evidence of the exchange of lipids between such {"}nanodiscs{"} and lipid monolayers adsorbed at either solid-liquid or air-liquid interfaces. This behavior has important implications for the potential uses of nanodiscs.",

author = "Gavin Hazell and Thomas Arnold and Barker, {Robert D.} and Clifton, {Luke A.} and Steinke, {Nina Juliane} and Cecilia Tognoloni and Edler, {Karen J.}",

year = "2016",

month = nov,

day = "15",

doi = "10.1021/acs.langmuir.6b02927",

language = "English",

volume = "32",

pages = "11845--11853",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "45",

}

TY - JOUR

T1 - Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems

AU - Hazell, Gavin

AU - Arnold, Thomas

AU - Barker, Robert D.

AU - Clifton, Luke A.

AU - Steinke, Nina Juliane

AU - Tognoloni, Cecilia

AU - Edler, Karen J.

PY - 2016/11/15

Y1 - 2016/11/15

N2 - Styrene-alt-maleic acid lipid particles (SMALPs) are self-assembled discoidal structures composed of a polymer belt and a segment of lipid bilayer, which are capable of encapsulating membrane proteins directly from the cell membrane. Here we present evidence of the exchange of lipids between such "nanodiscs" and lipid monolayers adsorbed at either solid-liquid or air-liquid interfaces. This behavior has important implications for the potential uses of nanodiscs.

AB - Styrene-alt-maleic acid lipid particles (SMALPs) are self-assembled discoidal structures composed of a polymer belt and a segment of lipid bilayer, which are capable of encapsulating membrane proteins directly from the cell membrane. Here we present evidence of the exchange of lipids between such "nanodiscs" and lipid monolayers adsorbed at either solid-liquid or air-liquid interfaces. This behavior has important implications for the potential uses of nanodiscs.

UR - http://www.scopus.com/inward/record.url?scp=84995790200&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1021/acs.langmuir.6b02927

U2 - 10.1021/acs.langmuir.6b02927

DO - 10.1021/acs.langmuir.6b02927

M3 - Article

AN - SCOPUS:84995790200

SN - 0743-7463

VL - 32

SP - 11845

EP - 11853

JO - Langmuir

JF - Langmuir

IS - 45

ER -

Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems

Abstract

Access to Document

Other files and links

Fingerprint

Detergent-free extraction and purification of membrane proteins to enable structural and functional studies.

Cite this

Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems

Abstract

Access to Document

Other files and links

Fingerprint

Projects

Detergent-free extraction and purification of membrane proteins to enable structural and functional studies.

Cite this