Evaluation of drug delivery to intact and porated skin by coherent Raman scattering and fluorescence microscopies

Natalie A. Belsey, Natalie L. Garrett, L. Rodrigo Contreras-Rojas, Adam J. Pickup-Gerlaugh, Gareth J. Price, Julian Moger, Richard H. Guy

Research output: Contribution to journalArticle

39 Citations (Scopus)
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Abstract

Stimulated Raman scattering microscopy was used to assess the permeation of topically applied drugs and formulation excipients into porcine skin. This chemically selective technique generates high-resolution 3D images, from which semi-quantitative information may be elucidated. Ibuprofen, applied as a close-to-saturated solution in propylene glycol, was directly observed to crystallise in/on the skin, as the co-solvent permeated more rapidly, resulting in precipitation of the drug. Coherent Raman scattering microscopy is also an excellent tool, in conjunction with more conventional confocal fluorescence microscopy, with which to image micro/nanoparticle-based formulations. Specifically, the uptake of particles into thermal ablation transport pathways in the skin has been examined.
Original languageEnglish
Pages (from-to)37-42
JournalJournal of Controlled Release
Volume174
Issue number28
Early online date11 Nov 2013
DOIs
Publication statusPublished - 28 Jan 2014

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Drug Evaluation
Raman Spectrum Analysis
Fluorescence Microscopy
Skin
Microscopy
Propylene Glycol
Drug Compounding
Ibuprofen
Excipients
Confocal Microscopy
Nanoparticles
Swine
Hot Temperature
Pharmaceutical Preparations

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Evaluation of drug delivery to intact and porated skin by coherent Raman scattering and fluorescence microscopies. / Belsey, Natalie A.; Garrett, Natalie L.; Contreras-Rojas, L. Rodrigo; Pickup-Gerlaugh, Adam J.; Price, Gareth J.; Moger, Julian; Guy, Richard H.

In: Journal of Controlled Release, Vol. 174, No. 28, 28.01.2014, p. 37-42.

Research output: Contribution to journalArticle

Belsey, Natalie A. ; Garrett, Natalie L. ; Contreras-Rojas, L. Rodrigo ; Pickup-Gerlaugh, Adam J. ; Price, Gareth J. ; Moger, Julian ; Guy, Richard H. / Evaluation of drug delivery to intact and porated skin by coherent Raman scattering and fluorescence microscopies. In: Journal of Controlled Release. 2014 ; Vol. 174, No. 28. pp. 37-42.
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