Establishment and Use of Mouse Haploid ES Cells

Martin Leeb, Tony Perry, Anton Wutz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Haploid genetics has facilitated new insights into mammalian pathways and disease mechanisms. Most animal cells are diploid, and mammalian haploid cell cultures have remained elusive for a long time. Recent methodological progress has enabled the routine derivation of haploid stem cell lines from mammalian haploid embryos. Here we provide detailed protocols for the establishment, culture, and manipulation of parthenogenetic and androgenetic haploid embryonic stem cells from mouse embryos.
LanguageEnglish
Pages155 - 185
Number of pages31
JournalCurrent Protocols in Mouse Biology
Volume5
Early online date1 Jun 2015
DOIs
StatusPublished - Jun 2015

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Haploidy
Mammalian Embryo
Diploidy
Stem Cells
Embryonic Structures
Cell Culture Techniques
Cell Line

Cite this

Establishment and Use of Mouse Haploid ES Cells. / Leeb, Martin; Perry, Tony; Wutz, Anton.

In: Current Protocols in Mouse Biology , Vol. 5, 06.2015, p. 155 - 185.

Research output: Contribution to journalArticle

Leeb, Martin ; Perry, Tony ; Wutz, Anton. / Establishment and Use of Mouse Haploid ES Cells. In: Current Protocols in Mouse Biology . 2015 ; Vol. 5. pp. 155 - 185.
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