Establishment and Use of Mouse Haploid ES Cells

Martin Leeb, Tony Perry, Anton Wutz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Haploid genetics has facilitated new insights into mammalian pathways and disease mechanisms. Most animal cells are diploid, and mammalian haploid cell cultures have remained elusive for a long time. Recent methodological progress has enabled the routine derivation of haploid stem cell lines from mammalian haploid embryos. Here we provide detailed protocols for the establishment, culture, and manipulation of parthenogenetic and androgenetic haploid embryonic stem cells from mouse embryos.
Original languageEnglish
Pages (from-to)155 - 185
Number of pages31
JournalCurrent Protocols in Mouse Biology
Volume5
Early online date1 Jun 2015
DOIs
Publication statusPublished - Jun 2015

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