ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

Luling Wu, Yang Wang, Maria Weber, Liyuan Liu, Adam C. Sedgwick, Steven D. Bull, Chusen Huang, Tony D. James

Research output: Contribution to journalArticlepeer-review

91 Citations (SciVal)


In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO- ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO-. ABAH-LW was able to detect low concentrations of ONOO- (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO- in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

Original languageEnglish
Pages (from-to)9953-9956
Number of pages4
JournalChemical Communications
Issue number71
Early online date7 Aug 2018
Publication statusE-pub ahead of print - 7 Aug 2018

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • General Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry


Dive into the research topics of 'ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite'. Together they form a unique fingerprint.

Cite this