ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

Luling Wu, Yang Wang, Maria Weber, Liyuan Liu, Adam C. Sedgwick, Steven D. Bull, Chusen Huang, Tony D. James

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO- ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO-. ABAH-LW was able to detect low concentrations of ONOO- (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO- in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

LanguageEnglish
Pages9953-9956
Number of pages4
JournalChemical Communications
Volume54
Issue number71
Early online date7 Aug 2018
DOIs
StatusE-pub ahead of print - 7 Aug 2018

Fingerprint

Peroxynitrous Acid
Fluorescent Dyes
Fluorescence
Imaging techniques
Experiments

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite. / Wu, Luling; Wang, Yang; Weber, Maria; Liu, Liyuan; Sedgwick, Adam C.; Bull, Steven D.; Huang, Chusen; James, Tony D.

In: Chemical Communications, Vol. 54, No. 71, 07.08.2018, p. 9953-9956.

Research output: Contribution to journalArticle

@article{1c19fa8be3ac49fca9f605a551c0b8a0,
title = "ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite",
abstract = "In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO- ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO-. ABAH-LW was able to detect low concentrations of ONOO- (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO- in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).",
author = "Luling Wu and Yang Wang and Maria Weber and Liyuan Liu and Sedgwick, {Adam C.} and Bull, {Steven D.} and Chusen Huang and James, {Tony D.}",
year = "2018",
month = "8",
day = "7",
doi = "10.1039/c8cc04919j",
language = "English",
volume = "54",
pages = "9953--9956",
journal = "Chemical communications (Cambridge, England)",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "71",

}

TY - JOUR

T1 - ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite

AU - Wu, Luling

AU - Wang, Yang

AU - Weber, Maria

AU - Liu, Liyuan

AU - Sedgwick, Adam C.

AU - Bull, Steven D.

AU - Huang, Chusen

AU - James, Tony D.

PY - 2018/8/7

Y1 - 2018/8/7

N2 - In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO- ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO-. ABAH-LW was able to detect low concentrations of ONOO- (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO- in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

AB - In this work, we set out to develop an endoplasmic reticulum (ER) directed ESIPT-based ONOO- ratiometric fluorescent probe (ABAH-LW). ABAH-LW was synthesized in four steps and found to have a high sensitivity and selectivity towards the detection of ONOO-. ABAH-LW was able to detect low concentrations of ONOO- (limit of detection = 21.4 nM) within seconds producing a ratiometric change in fluorescence intensity. ABAH-LW further demonstrated the ability to ratiometrically image endogenous and exogenous ONOO- in HeLa cells. Moreover, co-localization experiments were carried out using commercially available ER-Tracker Red, Lyso-Tracker Red and Mito-Tracker-Red, which were co-stained with ABAH-LW in HeLa cells. For ER-Tracker Red, Pearson's correlation co-efficient of 0.93 was determined and 3D surface plot analysis illustrated a large overlap between ABAH-LW and ER-Tracker Red using both red and blue channels. In addition some co-localisation with Mito-Tracker Red and ABAH-LW was observed (0.73).

UR - http://www.scopus.com/inward/record.url?scp=85052730200&partnerID=8YFLogxK

U2 - 10.1039/c8cc04919j

DO - 10.1039/c8cc04919j

M3 - Article

VL - 54

SP - 9953

EP - 9956

JO - Chemical communications (Cambridge, England)

T2 - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

SN - 1359-7345

IS - 71

ER -