Projects per year
Abstract
In the context of an integrated Lab-on-PCB wearable patch extracting interstitial fluid from the patient via integrated microneedles, the requirements from the integrated biosensing part are quite special compared to static glucose electrochemical biosensors. Hence, in this study, a fully PCB-integrated enzymatic glucose quantification Lab-on-Chip device is presented and evaluated considering these special requirements for such a patch implementation: a) range and limit of detection compatible with interstitial fluid glucose levels of diabetic patients and b) effect of sample flow rate on the biosensing platform performance. This work employs a chronoamperometric approach for glucose detection based on covalently immobilized glucose oxidase on PCB-integrated electrodes. The chronoamperometric measurements show that this platform exhibits μM range sensitivity, high specificity, and good reproducibility, and the assay can detect glucose from 10 μM to 9 mM with a lower limit of detection of 10 μM. The demonstrated detection range under continuous flow proved compatible with interstitial fluid glucose levels of diabetic patients. The sample-to-answer time of our Lab-on-PCB device is less than 1 min (sample delivery of few seconds and 20 s for electrochemical measurement), employing sample volumes of 50 μL in this instance. Increased flow rates substantially improve the platform sensitivity (1.1 μA/mM @0 μL/min to 6.2 μA/mM @10 μL/min), with the measured current increasing exponentially to the flow rate, as opposed to the theoretically expected much lower dependence. This work demonstrates the feasibility of Lab-on-PCB patches in terms of biosensing performance, paving the way for the first cost-effective, painless diabetes management microsystem.
Original language | English |
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Article number | 112484 |
Journal | Biosensors and Bioelectronics |
Volume | 167 |
Early online date | 5 Aug 2020 |
DOIs | |
Publication status | Published - 1 Nov 2020 |
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- 2 Finished
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Disposable self-powered selfcalibrated glucose biosensor for point of care diagnostics (SelfCare)
Moschou, D. (PI)
Engineering and Physical Sciences Research Council
26/03/18 → 25/09/18
Project: Research council
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Children-Friendly Patches Against an Emerging Diabetes Epidemic in Turkey
Moschou, D. (PI)
1/04/17 → 30/09/19
Project: Central government, health and local authorities