Enzymatic generation of short chain cello-oligosaccharides from Miscanthus using different pretreatments

Emanuele Kendrick, Rakesh Bhatia, Fernando C. Barbosa, Rosanna Goldbeck, Joe Gallagher, David Leak

Research output: Contribution to journalArticlepeer-review

4 Citations (SciVal)


Enzyme combinations producing short-chain cello-oligosaccharides (COS) as major bio-products from cellulose of Miscanthus Mx2779 accessed through different pretreatment methods were compared. Over short hydrolysis times, processive endoglucanase TfCel9a produced a high percentage of cellotetraose and cellopentaose and is synergistic with endoglucanase CcCel9m for producing short oligomers from amorphous cellulose but had low activity on untreated Miscanthus. Hydrolysis of the latter improved when these were combined with a mutant cellobio/triohydrolase OsCelC7(−105) and a lytic polysaccharide monooxygenase TrCel61a, a combination which also produced the highest COS yields from phosphoric acid swollen cellulose. Steam explosion pretreatment of Miscanthus increased COS yields, with/without phosphoric acid swelling, while increased swelling time (from 20 to 45 min) also increased yields but decreased the need for TrCel61a. The highest COS yields (933 mg/g glucan) and most stable product profile were obtained using ionic liquid [C 2mim][OAc] pretreatment and the three enzyme mixture TfCel9a, Cel9m and OsCel7a(−105).

Original languageEnglish
Article number127399
JournalBioresource Technology
Early online date29 May 2022
Publication statusPublished - 31 Aug 2022

Bibliographical note

Funding Information:
Funding: This work was supported by the Biotechnology and Biological Sciences Research BBSRC (UK) [grant number BB/P017460/1] and São Paulo Research Foundation, FAPESP (Brazil) [grant number 2015/50612-8]. In addition, the authors thank the BEACON Biorefining Centre for supportive work.

Link added to supplementary data.


  • Cello-oligosaccharides
  • Cellulose
  • Endoglucanases
  • Enzymatic hydrolysis
  • Lytic polysaccharide monooxygenase
  • Miscanthus
  • Pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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