Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

Pei Cao; Nasim Amiralian; Jingjing Wang; Bing Sun; Amirali Popat; Fengwei Xie; Zhi Ping Xu; Yan Li; Li Li

doi:10.1016/j.bioadv.2023.213400

Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

Pei Cao, Nasim Amiralian, Jingjing Wang, Bing Sun, Amirali Popat, Fengwei Xie, Zhi Ping Xu, Yan Li, Li Li

Research output: Contribution to journal › Article › peer-review

4 Citations (SciVal)

Abstract

Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.

Original language	English
Article number	213400
Journal	Biomaterials Advances
Volume	149
Early online date	24 Mar 2023
DOIs	https://doi.org/10.1016/j.bioadv.2023.213400
Publication status	Published - 1 Jun 2023

Bibliographical note

Publisher Copyright:
© 2023

Funding

The authors thank the financial support of the Queensland-Chinese Academy of Sciences Collaborative Science Fund (Q-CAS) and Advance Queensland Mid-Career Research Fellowship , Advance Queensland Research Fellowship and Chinese Scholarship Council (CSC). Thanks to the technical contribution of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis (CMM) and Australian National Fabrication Facility (ANFF-Q) at The University of Queensland. The authors acknowledge the Sugar Research Australia (SRA) for the supply of row sugarcane mulch for the extraction of nanocellulose. The authors thank the financial support of the Queensland-Chinese Academy of Sciences Collaborative Science Fund (Q-CAS) and Advance Queensland Mid-Career Research Fellowship, Advance Queensland Research Fellowship and Chinese Scholarship Council (CSC). Thanks to the technical contribution of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis (CMM) and Australian National Fabrication Facility (ANFF-Q) at The University of Queensland. The authors acknowledge the Sugar Research Australia (SRA) for the supply of row sugarcane mulch for the extraction of nanocellulose.

Funders	Funder number
Queensland-Chinese Academy of Sciences Collaborative Science Fund
Australian National Fabrication Facility
Sugar Research Australia
University of Queensland
China Scholarship Council
Advance Queensland

Keywords

Antigen internalization
Nano-cellulose composites
Oral vaccine delivery
Particle size
Polysaccharides
Stiffness

ASJC Scopus subject areas

Bioengineering
Biomaterials
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.bioadv.2023.213400

https://eprints.ncl.ac.uk/290517Licence: CC BY

Cite this

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title = "Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination",

abstract = "Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.",

keywords = "Antigen internalization, Nano-cellulose composites, Oral vaccine delivery, Particle size, Polysaccharides, Stiffness",

author = "Pei Cao and Nasim Amiralian and Jingjing Wang and Bing Sun and Amirali Popat and Fengwei Xie and Xu, {Zhi Ping} and Yan Li and Li Li",

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AU - Amiralian, Nasim

AU - Wang, Jingjing

AU - Sun, Bing

AU - Popat, Amirali

AU - Xie, Fengwei

AU - Xu, Zhi Ping

AU - Li, Yan

AU - Li, Li

PY - 2023/6/1

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N2 - Oral vaccine is a non-invasive, ideal way to protect communities from infectious diseases. Effective vaccine delivery systems are required to enhance vaccine absorption in the small intestine and its cellular uptake by immune cells. Here, we constructed alginate/chitosan-coated cellulose nanocrystal (Alg-Chi-CNC) and nanofibril (Alg-Chi-CNF) nanocomposites to enhance ovalbumin (OVA) delivery in the intestine. In vitro mucosal permeation and diffusion and cellular uptake demonstrated that Chi-CNC exhibited better cellular uptake in epithelial and antigen-presenting cells (APCs). In vivo results revealed that alginate/chitosan-coated nanocellulose nanocomposites generated strong systemic and mucosal immune responses. Though the features of functional nano-cellulose composites affected mucus permeation and APC uptakes, in vivo specific-OVA immune responses have not shown significant differences due to the complexity of the small intestine.

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Engineering nano-cellulose bio-composites to improve protein delivery for oral vaccination

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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Cite this