Engineering botulinum neurotoxin domains for activation by toxin light chain

P R Stancombe, Geoffrey Masuyer, I Birch-Machin, M Beard, K A Foster, J A Chaddock, K Ravi Acharya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Targeted secretion inhibitors (TSI) are a new class of biopharmaceuticals designed from a botulinum neurotoxin protein scaffold. The backbone consists of the 50-kDa endopeptidase light chain and translocation domain (N-terminal portion of the heavy chain), lacks neuronal toxicity, but retains the ability to target cytoplasmic soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. TSI are produced as single-chain proteins and then cleaved post-translationally to generate functional heterodimers. Precise proteolytic cleavage is essential to activate the protein to a dichain form. TSI are themselves highly specific proteases. We have exploited this activity to create self-activating enzymes by replacing the native proteolytic site with a substrate SNARE peptide for the TSI protease. We have also created cross-activating backbones. By replacing the proteolytic activation site in one backbone with the substrate SNARE peptide for another serotype, controlled activation is achieved. SNARE peptides encompassing the whole of the coiled-coil region enabled complete activation and assembly of the dichain backbone. These engineered TSI backbones are capable of translocating their enzymatic domains to target intracellular SNARE proteins. They are also investigative tools with which to further the understanding of endopeptidase activity of light chain in SNARE interactions.
Original languageEnglish
Pages (from-to)515-523
Number of pages9
JournalFEBS Journal
Volume279
Issue number3
DOIs
Publication statusPublished - Feb 2012

Keywords

  • targeted secretion inhibitors
  • LC endopeptidase domain
  • SNARE peptides
  • self-activation
  • botulinum toxin scaffold

Fingerprint Dive into the research topics of 'Engineering botulinum neurotoxin domains for activation by toxin light chain'. Together they form a unique fingerprint.

  • Cite this