Endothelin-converting enzyme 1 promotes re-sensitization of neurokinin 1 receptor-dependent neurogenic inflammation

F Cattaruzza, Graeme Cottrell, N Vaksman, N W Bunnett

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background and purpose:  The metalloendopeptidase endothelin-converting enzyme 1 (ECE-1) is prominently expressed in the endothelium where it converts big endothelin to endothelin-1, a vasoconstrictor peptide. Although ECE-1 is found in endosomes in endothelial cells, the role of endosomal ECE-1 is unclear. ECE-1 degrades the pro-inflammatory neuropeptide substance P (SP) in endosomes to promote recycling and re-sensitization of its neurokinin 1 (NK1) receptor. We investigated whether ECE-1 regulates NK1 receptor re-sensitization and the pro-inflammatory effects of SP in the endothelium.

Experimental approach:  We examined ECE-1 expression, SP trafficking and NK1 receptor re-sensitization in human microvascular endothelial cells (HMEC-1), and investigated re-sensitization of SP-induced plasma extravasation in rats.

Key results:  HMEC-1 expressed all four ECE-1 isoforms (a-d), and fluorescent SP trafficked to early endosomes containing ECE-1b/d. The ECE-1 inhibitor SM-19712 prevented re-sensitization of SP-induced Ca2+ signals in HMEC-1 cells. Immunoreactive ECE-1 and NK1 receptors co-localized in microvascular endothelial cells in the rat. SP-induced extravasation of Evans blue in the urinary bladder, skin and ears of the rat desensitized when the interval between two SP injections was 10 min, and re-sensitized after 480 min. SM-19712 inhibited this re-sensitization.

Conclusions and implications:  By degrading endocytosed SP, ECE-1 promotes the recycling and re-sensitization of NK1 receptors in endothelial cells, and thereby induces re-sensitization of the pro-inflammatory effects of SP. Thus, ECE-1 inhibitors may ameliorate the pro-inflammatory actions of SP.

Original languageEnglish
Pages (from-to)730-739
Number of pages10
JournalBritish Journal of Pharmacology
Volume156
Issue number5
DOIs
Publication statusPublished - Mar 2009

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Neurogenic Inflammation
Neurokinin-1 Receptors
Substance P
Endosomes
Endothelial Cells
Recycling
Enzyme Inhibitors
Endothelin-1
Endothelium
Endothelin-Converting Enzymes
Metalloendopeptidases
Evans Blue
Vasoconstrictor Agents
Endocytosis
Neuropeptides
Ear
Protein Isoforms
Urinary Bladder

Cite this

Endothelin-converting enzyme 1 promotes re-sensitization of neurokinin 1 receptor-dependent neurogenic inflammation. / Cattaruzza, F; Cottrell, Graeme; Vaksman, N; Bunnett, N W.

In: British Journal of Pharmacology, Vol. 156, No. 5, 03.2009, p. 730-739.

Research output: Contribution to journalArticle

Cattaruzza, F ; Cottrell, Graeme ; Vaksman, N ; Bunnett, N W. / Endothelin-converting enzyme 1 promotes re-sensitization of neurokinin 1 receptor-dependent neurogenic inflammation. In: British Journal of Pharmacology. 2009 ; Vol. 156, No. 5. pp. 730-739.
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abstract = "Background and purpose:  The metalloendopeptidase endothelin-converting enzyme 1 (ECE-1) is prominently expressed in the endothelium where it converts big endothelin to endothelin-1, a vasoconstrictor peptide. Although ECE-1 is found in endosomes in endothelial cells, the role of endosomal ECE-1 is unclear. ECE-1 degrades the pro-inflammatory neuropeptide substance P (SP) in endosomes to promote recycling and re-sensitization of its neurokinin 1 (NK1) receptor. We investigated whether ECE-1 regulates NK1 receptor re-sensitization and the pro-inflammatory effects of SP in the endothelium. Experimental approach:  We examined ECE-1 expression, SP trafficking and NK1 receptor re-sensitization in human microvascular endothelial cells (HMEC-1), and investigated re-sensitization of SP-induced plasma extravasation in rats. Key results:  HMEC-1 expressed all four ECE-1 isoforms (a-d), and fluorescent SP trafficked to early endosomes containing ECE-1b/d. The ECE-1 inhibitor SM-19712 prevented re-sensitization of SP-induced Ca2+ signals in HMEC-1 cells. Immunoreactive ECE-1 and NK1 receptors co-localized in microvascular endothelial cells in the rat. SP-induced extravasation of Evans blue in the urinary bladder, skin and ears of the rat desensitized when the interval between two SP injections was 10 min, and re-sensitized after 480 min. SM-19712 inhibited this re-sensitization. Conclusions and implications:  By degrading endocytosed SP, ECE-1 promotes the recycling and re-sensitization of NK1 receptors in endothelial cells, and thereby induces re-sensitization of the pro-inflammatory effects of SP. Thus, ECE-1 inhibitors may ameliorate the pro-inflammatory actions of SP.",
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