Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica

Stefanie Mühlhausen, Hans Dieter Schmitt, Kuan Ting Pan, Uwe Plessmann, Henning Urlaub, Laurence D. Hurst, Martin Kollmar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Although the “universal” genetic code is now known not to be universal, and stop codons can have multiple meanings, one regularity remains, namely that for a given sense codon there is a unique translation. Examining CUG usage in yeasts that have transferred CUG away from leucine, we here report the first example of dual coding: Ascoidea asiatica stochastically encodes CUG as both serine and leucine in approximately equal proportions. This is deleterious, as evidenced by CUG codons being rare, never at conserved serine or leucine residues, and predominantly in lowly expressed genes. Related yeasts solve the problem by loss of function of one of the two tRNAs. This dual coding is consistent with the tRNA-loss-driven codon reassignment hypothesis, and provides a unique example of a proteome that cannot be deterministically predicted. Video Abstract: [Figure presented] Mühlhausen et al. discover that Ascoidea asiatica stochastically encodes CUG as both serine and leucine, which is most likely caused by two competing tRNAs. This is the first example where the non-ambiguity rule of the genetic code is broken. To minimize its effect, A. asiatica uses CUG only rarely and never at conserved sequence positions.

LanguageEnglish
Pages2046-2057.e5
JournalCurrent Biology
Volume28
Issue number13
Early online date18 Jun 2018
DOIs
StatusPublished - 9 Jul 2018

Fingerprint

Ascoidea
RNA, Transfer, Ser
RNA, Transfer, Leu
codons
Codon
Leucine
leucine
Decoding
Transfer RNA
serine
Serine
Genetic Code
genetic code
Yeast
Yeasts
yeasts
Terminator Codon
conserved sequences
Conserved Sequence
stop codon

Keywords

  • Ascoidea asiatica
  • codon reassignment
  • competing tRNAs
  • CUG codon
  • genetic code
  • proteomics
  • Saccharomycopsis
  • stochastic decoding
  • yeast evolution

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Mühlhausen, S., Schmitt, H. D., Pan, K. T., Plessmann, U., Urlaub, H., Hurst, L. D., & Kollmar, M. (2018). Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica. Current Biology, 28(13), 2046-2057.e5. https://doi.org/10.1016/j.cub.2018.04.085

Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica. / Mühlhausen, Stefanie; Schmitt, Hans Dieter; Pan, Kuan Ting; Plessmann, Uwe; Urlaub, Henning; Hurst, Laurence D.; Kollmar, Martin.

In: Current Biology, Vol. 28, No. 13, 09.07.2018, p. 2046-2057.e5.

Research output: Contribution to journalArticle

Mühlhausen, S, Schmitt, HD, Pan, KT, Plessmann, U, Urlaub, H, Hurst, LD & Kollmar, M 2018, 'Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica', Current Biology, vol. 28, no. 13, pp. 2046-2057.e5. https://doi.org/10.1016/j.cub.2018.04.085
Mühlhausen, Stefanie ; Schmitt, Hans Dieter ; Pan, Kuan Ting ; Plessmann, Uwe ; Urlaub, Henning ; Hurst, Laurence D. ; Kollmar, Martin. / Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica. In: Current Biology. 2018 ; Vol. 28, No. 13. pp. 2046-2057.e5.
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