Abstract
The present invention relates to the use of one or more type 7 long terminal repeat (LTR7) nucleic acid sequences of type H human endogenous retroviruses (HERVH) ("LTR7/HERVH nucleic acid sequences") for identifying primate naive pluripotent stem cells. The invention is directed to the use of LTR7/HERVH nucleic acid sequences as a marker, wherein LTR7/HERVH-associated transcription is used as a marker for primate naive pluripotent stem cells. The invention also relates to a reporter construct comprising LTR7/HERVH nucleic acid sequences in addition to the use of said reporter, in particular for optimizing culture conditions for naïve primate pluripotent stem cells. The invention also relates to a cell growth medium for cultivation of primate naive pluripotent stem cells that preferably exhibit elevated levels of LTR7/HERVH-associated transcription in comparison to control cells.
| Original language | English |
|---|---|
| Patent number | WO 2016/055519 A1(EP3204490, US2017313978) |
| Priority date | 7/10/14 |
| Filing date | 7/10/15 |
| Publication status | Published - 2019 |
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MC2-Bioimaging and cell analysis
Material and Chemical Characterisation (MC2)Facility/equipment: Technology type