Abstract
This study presents experimental data of a fluidized-bed bioreactor for the cultivation of encapsulated pancreatic beta-cells. The fluidization quality for the bioreactor was evaluated at different flow rate using bed-expansion parameters. Homogeneous distribution of microcapsules was achieved at a flow rate of 2000 μL/min. This enabled efficient contact between the encapsulated cells and medium, which contributed to high cell viability. Microcapsule breakage was <4% on day 7 and confirmed the stability of encapsulated systems under fluidized culture. Importantly, endocrine beta-cells cultured in the bioreactor were shown to be dramatically more responsive to changes in glucose concentration compared to static culture (P < 0.001). On the basis of these results, cultivation of encapsulated cells in a fluidized bioreactor, especially for pancreatic beta-cells that are limited in supply, is a promising approach to address the lack of a safe method for storage and handling of cells between laboratories and clinical sites prior to transplantation.
Original language | English |
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Pages (from-to) | 1750-1757 |
Number of pages | 8 |
Journal | ACS Biomaterials Science and Engineering |
Volume | 3 |
Issue number | 8 |
Early online date | 19 Jun 2017 |
DOIs | |
Publication status | Published - 14 Aug 2017 |
Keywords
- bioreactor
- endocrine beta-cells
- fluidization
- insulin secretion
- microcapsules
- viability
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
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Marianne Ellis
- Department of Chemical Engineering - Professor
- Centre for Sustainable and Circular Technologies (CSCT)
- Institute for Policy Research (IPR)
- Centre for Digital, Manufacturing & Design (dMaDe)
Person: Research & Teaching, Core staff