Pseudomonas aeruginosa biofilm matrix polysaccharide Psl is regulated transcriptionally by RpoS and post-transcriptionally by RsmA

Yasuhiko Irie, Melissa Starkey, Adrianne N Edwards, Daniel J Wozniak, Tony Romeo, Matthew R Parsek

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Extracellular polysaccharides are important components of biofilms. In non-mucoid Pseudomonas aeruginosa strains, the Pel and Psl polysaccharides are major structural components of the biofilm matrix. In this study, we demonstrate that the alternative σ-factor RpoS is a positive transcriptional regulator of psl gene expression. Furthermore, we show that psl mRNA has an extensive 5' untranslated region, to which the post-transcriptional regulator RsmA binds and represses psl translation. Our observations suggest that upon binding RsmA, the region spanning the ribosome binding site of psl mRNA folds into a secondary stem-loop structure that blocks the Shine-Dalgarno sequence, preventing ribosome access and protein translation. This constitutes a novel mechanism for translational repression by this family of regulators.
Original languageEnglish
Pages (from-to)158-172
Number of pages15
JournalMolecular Microbiology
Volume78
Issue number1
DOIs
Publication statusPublished - Oct 2010

Keywords

  • 5' Untranslated Regions
  • Bacterial Proteins
  • Binding Sites
  • Biofilms
  • Gene Expression Regulation, Bacterial
  • Operon
  • Polysaccharides, Bacterial
  • Pseudomonas aeruginosa
  • RNA, Bacterial
  • RNA-Binding Proteins
  • Repressor Proteins
  • Sigma Factor
  • Transcription Initiation Site

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