Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma

Verónica Freire-Benéitez, Nicola Pomella, Thomas O Milner, Anaëlle Dumas, Maria Niklison Chirou, Eleni Maniati, Jun Wang, Vinothini Rajeeve, Pedro Cutillas, Silvia Marino

Research output: Contribution to journalArticlepeer-review

4 Citations (SciVal)

Abstract

Glioblastoma (GBM) is the most common and aggressive intrinsic brain tumour in adults. Epigenetic mechanisms controlling normal brain development are often dysregulated in GBM. Among these, BMI1, a structural component of the Polycomb Repressive Complex 1 (PRC1), which promotes the H2AK119ub catalytic activity of Ring1B, is upregulated in GBM and its tumorigenic role has been shown in vitro and in vivo. Here, we have used protein and chromatin immunoprecipitation followed by mass spectrometry (MS) analysis to elucidate the protein composition of PRC1 in GBM and transcriptional silencing of defining interactors in primary patient-derived GIC lines to assess their functional impact on GBM biology. We identify novel regulatory functions in mRNA splicing and cholesterol transport which could represent novel targetable mechanisms in GBM.
Original languageEnglish
Article numberzcab009
JournalNAR Cancer
Volume3
Issue number1
Early online date22 Mar 2021
DOIs
Publication statusPublished - 31 Mar 2021

Keywords

  • glioblastoma
  • BMI1

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