Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations

Paulo R F Rocha, Paul Schlett, Ulrike Kintzel, Volker Mailänder, Lode K J Vandamme, Gunther Zeck, Henrique L. Gomes, Fabio Biscarini, Dago M. De Leeuw

Research output: Contribution to journalArticle

20 Citations (Scopus)
60 Downloads (Pure)

Abstract

Microelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 μVpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance. This equivalent circuit leads to a Maxwell-Wagner relaxation frequency, the value of which is determined as a function of electrode area and molarity of an aqueous KCl electrolyte solution. The electrochemical voltage and current noise is measured as a function of electrode area and frequency and follow unambiguously from the measured impedance. By using large area electrodes the noise floor can be as low as 0.3 μVpp. The resulting high sensitivity is demonstrated by the extracellular detection of C6 glioma cell populations. Their minute electrical activity can be clearly detected at a frequency below about 10 Hz, which shows that the methodology can be used to monitor slow cooperative biological signals in cell populations.

Original languageEnglish
Article number34843
Pages (from-to)1-10
Number of pages10
JournalScientific Reports
Volume6
Early online date6 Oct 2016
DOIs
Publication statusPublished - 6 Oct 2016

Fingerprint

electrolytes
impedance
electrodes
equivalent circuits
background noise
potential fields
noise measurement
signal to noise ratios
capacitance
charge transfer
methodology
low frequencies
sensitivity
electric potential
cells
spectroscopy

ASJC Scopus subject areas

  • General

Cite this

Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations. / Rocha, Paulo R F; Schlett, Paul; Kintzel, Ulrike; Mailänder, Volker; Vandamme, Lode K J; Zeck, Gunther; Gomes, Henrique L.; Biscarini, Fabio; De Leeuw, Dago M.

In: Scientific Reports, Vol. 6, 34843, 06.10.2016, p. 1-10.

Research output: Contribution to journalArticle

Rocha, PRF, Schlett, P, Kintzel, U, Mailänder, V, Vandamme, LKJ, Zeck, G, Gomes, HL, Biscarini, F & De Leeuw, DM 2016, 'Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations', Scientific Reports, vol. 6, 34843, pp. 1-10. https://doi.org/10.1038/srep34843
Rocha, Paulo R F ; Schlett, Paul ; Kintzel, Ulrike ; Mailänder, Volker ; Vandamme, Lode K J ; Zeck, Gunther ; Gomes, Henrique L. ; Biscarini, Fabio ; De Leeuw, Dago M. / Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations. In: Scientific Reports. 2016 ; Vol. 6. pp. 1-10.
@article{10b338130c264d658dfe56074512f92b,
title = "Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations",
abstract = "Microelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 μVpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance. This equivalent circuit leads to a Maxwell-Wagner relaxation frequency, the value of which is determined as a function of electrode area and molarity of an aqueous KCl electrolyte solution. The electrochemical voltage and current noise is measured as a function of electrode area and frequency and follow unambiguously from the measured impedance. By using large area electrodes the noise floor can be as low as 0.3 μVpp. The resulting high sensitivity is demonstrated by the extracellular detection of C6 glioma cell populations. Their minute electrical activity can be clearly detected at a frequency below about 10 Hz, which shows that the methodology can be used to monitor slow cooperative biological signals in cell populations.",
author = "Rocha, {Paulo R F} and Paul Schlett and Ulrike Kintzel and Volker Mail{\"a}nder and Vandamme, {Lode K J} and Gunther Zeck and Gomes, {Henrique L.} and Fabio Biscarini and {De Leeuw}, {Dago M.}",
year = "2016",
month = "10",
day = "6",
doi = "10.1038/srep34843",
language = "English",
volume = "6",
pages = "1--10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations

AU - Rocha, Paulo R F

AU - Schlett, Paul

AU - Kintzel, Ulrike

AU - Mailänder, Volker

AU - Vandamme, Lode K J

AU - Zeck, Gunther

AU - Gomes, Henrique L.

AU - Biscarini, Fabio

AU - De Leeuw, Dago M.

PY - 2016/10/6

Y1 - 2016/10/6

N2 - Microelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 μVpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance. This equivalent circuit leads to a Maxwell-Wagner relaxation frequency, the value of which is determined as a function of electrode area and molarity of an aqueous KCl electrolyte solution. The electrochemical voltage and current noise is measured as a function of electrode area and frequency and follow unambiguously from the measured impedance. By using large area electrodes the noise floor can be as low as 0.3 μVpp. The resulting high sensitivity is demonstrated by the extracellular detection of C6 glioma cell populations. Their minute electrical activity can be clearly detected at a frequency below about 10 Hz, which shows that the methodology can be used to monitor slow cooperative biological signals in cell populations.

AB - Microelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 μVpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance. This equivalent circuit leads to a Maxwell-Wagner relaxation frequency, the value of which is determined as a function of electrode area and molarity of an aqueous KCl electrolyte solution. The electrochemical voltage and current noise is measured as a function of electrode area and frequency and follow unambiguously from the measured impedance. By using large area electrodes the noise floor can be as low as 0.3 μVpp. The resulting high sensitivity is demonstrated by the extracellular detection of C6 glioma cell populations. Their minute electrical activity can be clearly detected at a frequency below about 10 Hz, which shows that the methodology can be used to monitor slow cooperative biological signals in cell populations.

UR - http://www.scopus.com/inward/record.url?scp=84990842427&partnerID=8YFLogxK

UR - https://doi.org/10.1038/srep34843

UR - https://doi.org/10.1038/srep34843

U2 - 10.1038/srep34843

DO - 10.1038/srep34843

M3 - Article

VL - 6

SP - 1

EP - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 34843

ER -