Electrochemical Impedance Spectroscopy as a Tool for Monitoring Cell Differentiation from Floor Plate Progenitors to Midbrain Neurons in Real Time

Aya Elghajiji, Xin Wang, Stephen D. Weston, Guenther Zeck, Bastian Hengerer, David Tosh, Paulo R.F. Rocha

Research output: Contribution to journalArticlepeer-review

10 Citations (SciVal)

Abstract

Here shows that electrical impedance spectroscopy can be used as a non-invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The electrical data and equivalent circuit modeling are consistent with standard microscopy analysis and reveal that within the first 6 hours progenitor cells sediment and attach to the electrode within 40 hours. Between 40 and 120 hours, midbrain progenitor cells differentiate into midbrain neurons, followed by an electrochemically stable maturation phase. The ability to sense and characterize non-invasively and in real time cell differentiation opens up unprecedented avenues for implantable therapies and differentiation strategies.

Original languageEnglish
Article number2100330
JournalAdvanced Biology
Volume5
Issue number6
Early online date7 Apr 2021
DOIs
Publication statusPublished - 16 Jun 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors. Advanced Biology published by Wiley-VCH GmbH

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Funding

For technical support, the authors would like to acknowledge Dr. Michael Zachariadis, Dr. Sivapathasundaram Sivaraya, Michael Linham, and David Chapman at University of Bath, and Hans Peter Reich from the Max Planck Institute for Polymer Research, Mainz, Germany. The authors would also like to thank Dr Susanne Zach from Boehringer Ingelheim Pharma GmbH & Co. KG. P.R.F.R. acknowledges the support and funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No. 947897). A.E., P.R.F.R., and D.T. acknowledge funding from the Schlumberger Foundation Faculty for the Future Fellowship.

Keywords

  • bioelectronics
  • biophysics
  • cell differentiation
  • electrochemical impedance spectroscopy
  • spinal cord injuries

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • Biomedical Engineering
  • Biomaterials

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