Electrochemical Glucose Biosensor with Commercially Fabricated Printed Circuit Board Sensing Electrodes: The Importance of Electrode Surface Characteristics in Sensor Sensitivity

Diabetes mellitus is an important chronic disease affecting increasing population numbers. The World Health Organization (WHO) has placed diabetes among the top 10 world diseases with highest mortality rates. Our main goal is to develop a low-cost upscalable biosensor device integrated with sample extraction and delivery microfluidics via Printed-Circuit-Board (PCB) technology for Glucose quantification. Biosensors implemented with PCB are emerging as an attractive alternative to the µTAS commercial upscaling bottleneck, as they offer an affordable and standardized manufacturing solution for integrated microfluidic systems. Here we report the first PCB-implemented electrochemical glucose biosensor using covalently immobilized glucose oxidase (GOx) on the PCB surface taking particular care on the electrode surface characteristics and their effect on sensor performance.