Efficient metaphase II transgenesis with different transgene archetypes

Anthony C F Perry, A Rothman, J I de las Heras, P Feinstein, P Mombaerts, H J Cooke, T Wakayama

Research output: Contribution to journalArticle

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Abstract

Mammalian genome characterization and biotechnology each require the mobilization of large DNA segments to produce transgenic animals. We recently showed that mouse metaphase II (mll) oocytes could efficiently promote transgenesis (mll transgenesis) when coinjected with sperm and small (< 5 kilobases) ubiquitously expressed transgenes (tgs)(1). We have extended this work and now report that mll transgenesis can readily be applied to a range of larger tgs (11.9-170 kilobases), including bacterial and mammalian artificial chromosome (BAC and MAC) constructs. The efficiency of large-construct mll transgenesis was at least as high as that with small constructs; 11-47% of offspring carried the large tgs. More than 95% of these transgenic founders transmitted the tg to offspring. These data demonstrate the ability of mll transgenesis to deliver large tgs efficiently.
LanguageEnglish
Pages1071-1073
Number of pages3
JournalNature Biotechnology
Volume19
Issue number11
DOIs
StatusPublished - 2001

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Gene Transfer Techniques
Metaphase
Biotechnology
Chromosomes
Transgenes
Animals
DNA
Genes
Mammalian Artificial Chromosomes
Bacterial Artificial Chromosomes
Genetically Modified Animals
Oocytes
Spermatozoa
Genome

Cite this

Perry, A. C. F., Rothman, A., de las Heras, J. I., Feinstein, P., Mombaerts, P., Cooke, H. J., & Wakayama, T. (2001). Efficient metaphase II transgenesis with different transgene archetypes. Nature Biotechnology, 19(11), 1071-1073. https://doi.org/10.1038/nbt1101-1071

Efficient metaphase II transgenesis with different transgene archetypes. / Perry, Anthony C F; Rothman, A; de las Heras, J I; Feinstein, P; Mombaerts, P; Cooke, H J; Wakayama, T.

In: Nature Biotechnology, Vol. 19, No. 11, 2001, p. 1071-1073.

Research output: Contribution to journalArticle

Perry, ACF, Rothman, A, de las Heras, JI, Feinstein, P, Mombaerts, P, Cooke, HJ & Wakayama, T 2001, 'Efficient metaphase II transgenesis with different transgene archetypes', Nature Biotechnology, vol. 19, no. 11, pp. 1071-1073. https://doi.org/10.1038/nbt1101-1071
Perry ACF, Rothman A, de las Heras JI, Feinstein P, Mombaerts P, Cooke HJ et al. Efficient metaphase II transgenesis with different transgene archetypes. Nature Biotechnology. 2001;19(11):1071-1073. https://doi.org/10.1038/nbt1101-1071
Perry, Anthony C F ; Rothman, A ; de las Heras, J I ; Feinstein, P ; Mombaerts, P ; Cooke, H J ; Wakayama, T. / Efficient metaphase II transgenesis with different transgene archetypes. In: Nature Biotechnology. 2001 ; Vol. 19, No. 11. pp. 1071-1073.
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