Effects of common sterilization methods on the structure and properties of poly(D,L lactic-co-glycolic acid) scaffolds

Holly Shearer, Marianne J Ellis, Semali P Perera, Julian B Chaudhuri

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

While methods for the prodn. of scaffolds with the appropriate mech. properties and architecture for tissue engineering are attracting much attention, the effects of subsequent sterilization processes on the scaffold properties have often been overlooked. This study sought to det. the effects of sterilization with ethanol, peracetic acid, UV irradn., and antibiotic soln. on the structure of 50:50 (mol:mol) 65:35, and 85:15 poly(D,L-lactic-co-glycolic acid [PLGA]) flat-sheet and hollow-fiber scaffolds. All methods resulted in scaffold sterilization, but SEM revealed deformations to the scaffold surface for all treatments. The extent of surface damage increased with treatment duration. This was further investigated by measurement of pore sizes, water flux, breaking strain, and Young's modulus. External pore size and water flux was found to be increased by all treatments in the following order: ethanol (largest), antibiotics, UV light, and peracetic acid. Pore sizes were 0.25 to 0.17 micro m and water flux ranged from 0.01 kg m-2 s-1 to 3.34 kg m-2 s-1. For all samples, the Young's modulus was 1.0 to 31.1MPa and breaking strain was 1.2 to 2.4 MPa. The results of this study suggest that antibiotic treatment shows the most potential to sterilize PLGA hollow fibers for tissue engineering. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)2717-2727
Number of pages11
JournalTissue Engineering
Volume12
Issue number10
Publication statusPublished - 2006

Keywords

  • sterilization ethanol peracetic acid UV irradn antibiotic tissue engineering
  • Prosthetic materials and Prosthetics (polymers
  • antibiotic but not ethanol
  • UV radiation (antibiotic but not UV irradn. showed most potential to sterilize poly(D
  • Antibiotics
  • peracetic acid
  • unclassified)
  • BIOL (Biological study)
  • Tissue engineering (antibiotic but not ethanol
  • BSU (Biological study
  • THU (Therapeutic use)
  • poly D L lactic co glycolic acid scaffold
  • Sterilization and Disinfection
  • USES (Uses) (dilactone-based
  • Polyesters Role
  • L-lactic-co-glycolic acid) hollow fibers for tissue engineering but all treatment damaged surface of scaffolds)
  • UV irradn. showed most potential to sterilize poly(D

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