Effects of C-17 heterocyclic substituents on the anticancer activity of 2-ethylestra-1,3,5(10)-triene-3-O-sulfamates: synthesis, in vitro evaluation and computational modelling

Fabrice Jourdan, Christian Bubert, Mathew P. Leese, Andrew Smith, Eric Ferrandis, Sandra Regis-Lydi, Simon P. Newman, Atul Purohit, Michael J. Reed, Barry V. L. Potter

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Abstract

The potent activity of 2-substituted estra-1,3,5(10)-triene-3-O-sulfamates against the proliferation of cancer cells in vitro and tumours in vivo highlights the therapeutic potential of such compounds. Optimal activity is derived from a combination of a 2-XMe group ( where X = CH2, O or S), a 3-O-sulfamate group in the steroidal A-ring and a H-bond acceptor around C-17 of the D-ring. Herein, we describe the synthesis and anti-proliferative activities of a series of novel 2-substituted estra-1,3,5( 10)-triene-3-O-sulfamates bearing heterocyclic substituents ( oxazole, tetrazole, triazole) tethered to C-17. In vitro evaluation of these molecules revealed that high anti-proliferative activity in breast and prostate cancer cells lines (GI(50) of 340-850 nM) could be retained when the heterocyclic substituent possesses H-bond acceptor properties. A good correlation between the calculated electron density of the heterocyclic ring and anti-proliferative activity was observed. Docking of the most active compounds into their putative site of action, the colchicine binding site of tubulin, suggests that they bind through a different mode to the previously described bis-sulfamate derivatives 1 and 2, which possess similar in vitro activity.
Original languageEnglish
Pages (from-to)4108-4119
JournalOrganic and Biomolecular Chemistry
Volume6
Issue number22
DOIs
Publication statusPublished - 2008

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