Effect of poly (ADP-ribose) polymerase-1 inhibition on the proliferation of murine colon carcinoma CT26 cells

L Cai, M D Threadgill, Y L Wang, M Li

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To investigate effect of poly (ADP-ribose) polymerase inhibition on the proliferation of CT26 cells in vitro and the mechanism of this effect. CT26 cells were treated with a range of concentrations of 5-Aminoisoquinolin-1-one (PARP inhibitor) in vitro. MTT assays and flow cytometry were used to determine the proliferation and cell cycle distribution, respectively, of the cells. The expression of PARP-1 was investigated by Western blot. The binding of Nuclear Factor-kappa B to DNA was detected by electrophoretic mobility shift assay. The proliferation of CT26 cells was significantly inhibited by 5-AIQ induced PARP inhibition in a dose-dependent manner. Proliferation was inhibited by 17.5% at 100 mu M 5-AIQ, by 27.6% at 500 mu M 5-AIQ and by 39.9% at 1000 mu M (P
Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalPathology & Oncology Research
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 2009

Keywords

  • NF-kappa B
  • Proliferation
  • PARP-1
  • Colon carcinoma
  • In vitro

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