Effect of contact time and inhibitor concentration on the affinity mediated adsorption of cells to surfaces

A Lam, X Cao, R Eisenthal, J Hubble

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Cell detachment by shear stress under conditions of laminar flow was used to investigate the effect of incubation time and sol. binding competitors on affinity mediated cell/surface interactions. Fractional attachment between yeast and a Con A coated surface was studied as a function of adhesion time prior to exposure to shear in a parallel plate flow chamber. Two, four and sixteen hours adhesion times gave rise to significantly different fractional attachment profiles, with four hours giving greater cell retention. The effect of dextran as a competitive displacer of pre-attached cells was also examd. using a no. of exposure regimes. While the presence of dextran in the displacement buffer led to higher fractional displacement of pre-attached cells, this effect was magnified if an equilibration period between dextran soln. and pre-attached cells was allowed before detachment was attempted. The decline in fractional attachment increased with incubation time up to 30 min, with longer periods resulting in a smaller effect. Pre-incubation of the Con A surface with dextran prior to the introduction of cells led to a 60% redn. in attachment. Attempts to det. crit. shear values were complicated by the presence of a tightly bound cell fraction of approx. 15% that was not removed at the highest shear values used. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalEnzyme Microbial Technology
Volume29
Issue number1
Publication statusPublished - 2001

Keywords

  • Shear stress
  • Yeast (effect of contact time and inhibitor concn. on affinity mediated adsorption of cells to surfaces)
  • effect of contact time and inhibitor concn. on affinity mediated adsorption of cells to surfaces)
  • Adsorption
  • yeast cell adsorption surface shear stress
  • Adhesion
  • Flow (laminar

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