Effect of Carbohydrate-Protein Supplementation on Endurance Training Adaptations: Post-exercise protein ingestion and endurance training

Abdullah F. Alghannam, Iain Templeman, Joel Thomas, Dawid Jedrzejewski, Samuel Griffiths, Joseph Lemon, Thomas Byers, Sue Reeves, Javier Gonzalez, Dylan Thompson, James Bilzon, Kostas Tsintzas, James Betts

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Purpose: To examine the influence of post-exercise protein feeding upon the adaptive response to endurance exercise training. Methods: In a randomised parallel group design, 25 healthy men and women completed 6 weeks of endurance exercise training by running on a treadmill for 30–60 min at 70–75% maximal oxygen uptake (VO 2max) 4 times/week. Participants ingested 1.6 g per kilogram of body mass (g kg BM −1) of carbohydrate (CHO) or an isocaloric carbohydrate–protein solution (CHO-P; 0.8 g carbohydrate kg BM −1 + 0.8 g protein kg BM −1) immediately and 1 h post-exercise. Expired gas, blood and muscle biopsy samples were taken at baseline and follow-up. Results: Exercise training improved VO 2max in both groups (p ≤ 0.001), but this increment was not different between groups either in absolute terms or relative to body mass (0.2 ± 0.2 L min −1 and 3.0 ± 2 mL kg −1 min −1, respectively). No change occurred in plasma albumin concentration from baseline to follow-up with CHO-P (4.18 ± 0.18 to 4.23 ± 0.17 g dL −1) or CHO (4.17 ± 0.17 to 4.12 ± 0.22 g dL −1; interaction: p > 0.05). Mechanistic target of rapamycin (mTOR) gene expression was up-regulated in CHO-P (+ 46%; p = 0.025) relative to CHO (+ 4%) following exercise training. Conclusion: Post-exercise protein supplementation up-regulated the expression of mTOR in skeletal muscle over 6 weeks of endurance exercise training. However, the magnitude of improvement in VO 2max was similar between groups.

Original languageEnglish
Pages (from-to)2273-2287
JournalEuropean Journal of Applied Physiology
Volume120
Early online date5 Aug 2020
DOIs
Publication statusPublished - 31 Oct 2020

Keywords

  • Amino acids
  • Post-exercise nutrition
  • Recovery
  • Running
  • Sucrose

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)

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