Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial

Harriet Amy Carroll, Iain Templeman, Yung-chih Chen, Robert M. Edinburgh, Elaine K. Burch, Jake T. Jewitt, Georgie Povey, Timothy D. Robinson, William L. Dooley, Robert Jones, Kostas Tsintzas, Widet Gallo, Olle Melander, Dylan Thompson, Lewis John James, Laura Johnson, James A. Betts

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Abstract

The aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 male) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated, after four days of pre-trial standardization. One day pre-OGTT, participants were dehydrated for 1-h in a heat-tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measures and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken pre- and post-intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9±1.2% body mass loss, 2.9±2.7% cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all p≤0.007). Fasted serum glucose (HYPO 5.10±0.42 mmol∙l‑1; RE 5.02±0.40 mmol∙l-1; p=0.327) and insulin (HYPO 27.1±9.7 pmol∙L-1; RE 27.6±9.2 pmol∙L-1; p=0.809) concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose ( p=0.627) or insulin ( p=0.200) responses during the OGTT. Muscle water content was lower pre-OGTT after HYPO compared to RE (761±13 g∙kg-1 wet weight versus 772±18 g∙kg-1 RE), but similar post-OGTT (HYPO 779±15 g∙kg-1 versus RE 780±20 g∙kg‑1; time p=0.011; trial*time p = 0.055). Resting energy expenditure was similar between hydration states (stable between -1.21 and 5.94 kJ∙kg-1∙d-1; trial p=0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.
Original languageEnglish
Pages (from-to)422-430
JournalJournal of Applied Physiology
Volume126
Issue number2
Early online date20 Feb 2019
DOIs
Publication statusPublished - 28 Feb 2019

Cite this

Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial. / Carroll, Harriet Amy; Templeman, Iain; Chen, Yung-chih; Edinburgh, Robert M.; Burch, Elaine K.; Jewitt, Jake T.; Povey, Georgie; Robinson, Timothy D.; Dooley, William L.; Jones, Robert; Tsintzas, Kostas; Gallo, Widet; Melander, Olle; Thompson, Dylan; James, Lewis John; Johnson, Laura; Betts, James A.

In: Journal of Applied Physiology, Vol. 126, No. 2, 28.02.2019, p. 422-430.

Research output: Contribution to journalArticle

Carroll, HA, Templeman, I, Chen, Y, Edinburgh, RM, Burch, EK, Jewitt, JT, Povey, G, Robinson, TD, Dooley, WL, Jones, R, Tsintzas, K, Gallo, W, Melander, O, Thompson, D, James, LJ, Johnson, L & Betts, JA 2019, 'Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial', Journal of Applied Physiology, vol. 126, no. 2, pp. 422-430. https://doi.org/10.1152/japplphysiol.00771.2018
Carroll, Harriet Amy ; Templeman, Iain ; Chen, Yung-chih ; Edinburgh, Robert M. ; Burch, Elaine K. ; Jewitt, Jake T. ; Povey, Georgie ; Robinson, Timothy D. ; Dooley, William L. ; Jones, Robert ; Tsintzas, Kostas ; Gallo, Widet ; Melander, Olle ; Thompson, Dylan ; James, Lewis John ; Johnson, Laura ; Betts, James A. / Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial. In: Journal of Applied Physiology. 2019 ; Vol. 126, No. 2. pp. 422-430.
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AU - Chen, Yung-chih

AU - Edinburgh, Robert M.

AU - Burch, Elaine K.

AU - Jewitt, Jake T.

AU - Povey, Georgie

AU - Robinson, Timothy D.

AU - Dooley, William L.

AU - Jones, Robert

AU - Tsintzas, Kostas

AU - Gallo, Widet

AU - Melander, Olle

AU - Thompson, Dylan

AU - James, Lewis John

AU - Johnson, Laura

AU - Betts, James A.

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N2 - The aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 male) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated, after four days of pre-trial standardization. One day pre-OGTT, participants were dehydrated for 1-h in a heat-tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measures and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken pre- and post-intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9±1.2% body mass loss, 2.9±2.7% cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all p≤0.007). Fasted serum glucose (HYPO 5.10±0.42 mmol∙l‑1; RE 5.02±0.40 mmol∙l-1; p=0.327) and insulin (HYPO 27.1±9.7 pmol∙L-1; RE 27.6±9.2 pmol∙L-1; p=0.809) concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose ( p=0.627) or insulin ( p=0.200) responses during the OGTT. Muscle water content was lower pre-OGTT after HYPO compared to RE (761±13 g∙kg-1 wet weight versus 772±18 g∙kg-1 RE), but similar post-OGTT (HYPO 779±15 g∙kg-1 versus RE 780±20 g∙kg‑1; time p=0.011; trial*time p = 0.055). Resting energy expenditure was similar between hydration states (stable between -1.21 and 5.94 kJ∙kg-1∙d-1; trial p=0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.

AB - The aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 male) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated, after four days of pre-trial standardization. One day pre-OGTT, participants were dehydrated for 1-h in a heat-tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measures and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken pre- and post-intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9±1.2% body mass loss, 2.9±2.7% cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all p≤0.007). Fasted serum glucose (HYPO 5.10±0.42 mmol∙l‑1; RE 5.02±0.40 mmol∙l-1; p=0.327) and insulin (HYPO 27.1±9.7 pmol∙L-1; RE 27.6±9.2 pmol∙L-1; p=0.809) concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose ( p=0.627) or insulin ( p=0.200) responses during the OGTT. Muscle water content was lower pre-OGTT after HYPO compared to RE (761±13 g∙kg-1 wet weight versus 772±18 g∙kg-1 RE), but similar post-OGTT (HYPO 779±15 g∙kg-1 versus RE 780±20 g∙kg‑1; time p=0.011; trial*time p = 0.055). Resting energy expenditure was similar between hydration states (stable between -1.21 and 5.94 kJ∙kg-1∙d-1; trial p=0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.

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