Dysfunction of endothelial progenitor cells is associated with the type I IFN pathway in patients with polymyositis and dermatomyositis

L Ekholm, J Michelle Kahlenberg, Sevim Barbasso Helmers, Anna Tjarnlund, Srilakshmi Yalavarthi, Wenpu Zhao, Nickie Seto, Zoe Betteridge, Ingrid E Lundberg, Mariana J Kaplan

Research output: Contribution to journalArticle

11 Citations (Scopus)
42 Downloads (Pure)

Abstract

Objective. Alterations in phenotype and function of endothelial progenitor cells (EPCs) have been associated with poor vascular outcomes and impaired vascular repair in various conditions. Our hypothesis was that patients with PM and DM have dysregulation of EPCs driven by type I IFN and IL-18 similar to other autoimmune diseases.

Methods. Quantification of circulating EPCs was performed by flow cytometry in patients with PM/DM and matched healthy controls. The ability of EPCs to differentiate into mature endothelial cells was investigated by light and fluorescence microscopy quantification in the presence or absence of PM/DM or control serum, neutralizing antibodies to type I IFN receptor or IL-18. Serum type I IFN activity was quantified by induction of type I IFN-inducible genes in HeLa cells. Circulating IL-18 concentrations were assessed by ELISA.

Results. Circulating EPCs were significantly lower in PM/DM patients compared with controls. PM/DM EPCs displayed a decreased capacity to differentiate into mature endothelial cells and PM/DM serum significantly inhibited differentiation of control EPCs. This effect was reversed in the majority of samples with neutralizing antibodies to IL-18 or to type I IFN receptor or by a combination of these antibodies. Patients with associated impairments in EPC function had higher type I IFN serum activity.

Conclusion. PM/DM is associated with dysregulation of EPC phenotype and function that may be attributed, at least in part, to aberrant IL-18 and type I IFN pathways. The implication of these vasculopathic findings for disease prognosis and complications remains to be determined.
Original languageEnglish
Pages (from-to)1987-1992
JournalRheumatology
Volume55
Issue number11
Early online date7 Aug 2016
DOIs
Publication statusPublished - Nov 2016

Fingerprint Dive into the research topics of 'Dysfunction of endothelial progenitor cells is associated with the type I IFN pathway in patients with polymyositis and dermatomyositis'. Together they form a unique fingerprint.

  • Cite this

    Ekholm, L., Kahlenberg, J. M., Helmers, S. B., Tjarnlund, A., Yalavarthi, S., Zhao, W., Seto, N., Betteridge, Z., Lundberg, I. E., & Kaplan, M. J. (2016). Dysfunction of endothelial progenitor cells is associated with the type I IFN pathway in patients with polymyositis and dermatomyositis. Rheumatology, 55(11), 1987-1992. https://doi.org/10.1093/rheumatology/kew288