Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Emma V. Lampard, Adam C. Sedgwick, Thitima Sombuttan, George T. Williams, Boontana Wannalerse, A. Toby A. Jenkins, Steven D. Bull, Tony D. James

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

Original languageEnglish
Pages (from-to)266-268
Number of pages3
JournalChemistryOpen
Volume7
Issue number3
Early online date30 Jan 2018
DOIs
Publication statusPublished - 1 Mar 2018

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Keywords

  • boronic acids
  • colorimetric detection
  • dye displacement assays
  • glucose
  • saccharides

ASJC Scopus subject areas

  • Chemistry(all)

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