Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Emma V. Lampard; Adam C. Sedgwick; Thitima Sombuttan; George T. Williams; Boontana Wannalerse; A. Toby A. Jenkins; Steven D. Bull; Tony D. James

doi:10.1002/open.201700193

Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Emma V. Lampard, Adam C. Sedgwick, Thitima Sombuttan, George T. Williams, Boontana Wannalerse, A. Toby A. Jenkins, Steven D. Bull, Tony D. James

Kasetsart University

Research output: Contribution to journal › Article › peer-review

12 Citations (SciVal)

Abstract

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

Original language	English
Pages (from-to)	266-268
Number of pages	3
Journal	ChemistryOpen
Volume	7
Issue number	3
Early online date	30 Jan 2018
DOIs	https://doi.org/10.1002/open.201700193
Publication status	Published - 1 Mar 2018

Keywords

boronic acids
colorimetric detection
dye displacement assays
glucose
saccharides

ASJC Scopus subject areas

General Chemistry

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10.1002/open.201700193Licence: CC BY

Cite this

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title = "Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels",

abstract = "Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.",

keywords = "boronic acids, colorimetric detection, dye displacement assays, glucose, saccharides",

author = "Lampard, {Emma V.} and Sedgwick, {Adam C.} and Thitima Sombuttan and Williams, {George T.} and Boontana Wannalerse and Jenkins, {A. Toby A.} and Bull, {Steven D.} and James, {Tony D.}",

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T1 - Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

AU - Lampard, Emma V.

AU - Sedgwick, Adam C.

AU - Sombuttan, Thitima

AU - Williams, George T.

AU - Wannalerse, Boontana

AU - Jenkins, A. Toby A.

AU - Bull, Steven D.

AU - James, Tony D.

PY - 2018/3/1

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N2 - Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

AB - Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

KW - boronic acids

KW - colorimetric detection

KW - dye displacement assays

KW - glucose

KW - saccharides

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EP - 268

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JF - ChemistryOpen

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ER -

Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Abstract

Keywords

ASJC Scopus subject areas

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Electrospray Time-of-Flight Mass Spectrometer (Open-Access)

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Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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Electrospray Time-of-Flight Mass Spectrometer (Open-Access)

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