Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels

Emma V. Lampard, Adam C. Sedgwick, Thitima Sombuttan, George T. Williams, Boontana Wannalerse, A. Toby A. Jenkins, Steven D. Bull, Tony D. James

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid–Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.

Original languageEnglish
Pages (from-to)266-268
JournalChemistryOpen
Volume7
Issue number3
Early online date30 Jan 2018
DOIs
Publication statusPublished - 1 Mar 2018

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Hydrogels
Assays
Coloring Agents
Boronic Acids
Monosaccharides
Fructose
Esters
Glucose

Cite this

Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels. / Lampard, Emma V.; Sedgwick, Adam C.; Sombuttan, Thitima; Williams, George T.; Wannalerse, Boontana; Jenkins, A. Toby A.; Bull, Steven D.; James, Tony D.

In: ChemistryOpen, Vol. 7, No. 3, 01.03.2018, p. 266-268.

Research output: Contribution to journalArticle

Lampard, Emma V. ; Sedgwick, Adam C. ; Sombuttan, Thitima ; Williams, George T. ; Wannalerse, Boontana ; Jenkins, A. Toby A. ; Bull, Steven D. ; James, Tony D. / Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels. In: ChemistryOpen. 2018 ; Vol. 7, No. 3. pp. 266-268.
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