Dual-specific interaction to detect DNA on gold nanoparticles

C.-L. Feng, X.-Q. Dou, Q.-L. Liu, W. Zhang, J.-J. Gu, S.-M. Zhu, A Toby A Jenkins, D. Zhang

Research output: Contribution to journalArticlepeer-review

3 Citations (SciVal)
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An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as "bridges" to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
Original languageEnglish
Pages (from-to)5749-5756
Number of pages8
Issue number5
Publication statusPublished - 1 Dec 2013


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