Dual-specific interaction to detect DNA on gold nanoparticles

C.-L. Feng; X.-Q. Dou; Q.-L. Liu; W. Zhang; J.-J. Gu; S.-M. Zhu; A Toby A Jenkins; D. Zhang

doi:10.3390/s130505749

Dual-specific interaction to detect DNA on gold nanoparticles

C.-L. Feng, X.-Q. Dou, Q.-L. Liu, W. Zhang, J.-J. Gu, S.-M. Zhu, A Toby A Jenkins, D. Zhang

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Abstract

An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as "bridges" to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.

Original language	English
Pages (from-to)	5749-5756
Number of pages	8
Journal	Sensors
Volume	13
Issue number	5
DOIs	https://doi.org/10.3390/s130505749
Publication status	Published - 1 Dec 2013

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10.3390/s130505749

Sensors 13 05749
© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Cite this

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AU - Zhu, S.-M.

AU - Jenkins, A Toby A

AU - Zhang, D.

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AB - An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as "bridges" to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.

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Dual-specific interaction to detect DNA on gold nanoparticles

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