Dual enzyme activated fluorescein based fluorescent probe

Maria L. Odyniec, Jordan E. Gardiner, Adam C. Sedgwick, Xiao Peng He, Steven D. Bull, Tony D. James

Research output: Contribution to journalArticlepeer-review

13 Citations (SciVal)


A simple dual analyte fluorescein-based probe (PF3-Glc) was synthesised containing β-glucosidase (β-glc) and hydrogen peroxide (H 2 O 2 ) trigger units. The presence of β-glc, resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein (PF3). Subsequently, in the presence of glucose oxidase (GOx), the released glucose was catalytically converted to D-glucono-δ-lactone, which produced H 2 O 2 as a by-product. The GOx-produced H 2 O 2 , resulted in classic H 2 O 2 -mediated boronate oxidation and the release of the highly emissive fluorophore, fluorescein. This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.[Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)117–121
JournalFrontiers of Chemical Science and Engineering
Early online date1 Mar 2019
Publication statusPublished - 1 Feb 2020


  • chemosensors
  • dual-activation
  • fluorescence
  • GOx
  • molecular logic
  • β-glucosidase

ASJC Scopus subject areas

  • General Chemical Engineering


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