dsDNA modified carbon nanofiber-solidified paste electrodes: probing Ni(II)-dsDNA interactions

A Ferancova, Liza Rassaei, Frank Marken, J Labuda, M Sillanpaa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to develop a renewable electrode surface, carbon nanofibers (CNF) were embedded into solidified paste electrodes using a composite of paraffin wax and paraffin oil. A range of different compositions was surveyed and the optimal composition of the paste for electroanalysis was found to be 43% of CNF, 41% of paraffin wax, and 16% of paraffin oil. The electrochemical properties of the novel composite electrode were investigated using cyclic voltammetry and electrochemical impedance spectroscopy and compared to those of similar graphite-solidified paste electrodes. The carbon nanofibers enhance the activity of the surface of the electrode and provide a good substrate for the adsorption and voltammetric detection of dsDNA. Responses of dsDNA bases and Ni2+ ions accumulated from ammonium buffer pH 8.5 (with a Langmuirian binding constant of 10(5) mol(-1) L) were investigated and a limit of detection of 7 nmol L-1 (at 3 sigma) was obtained using "nucleation stripping voltammetry". Interferences by other metal cations are examined and discussed.
Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalMicrochimica Acta
Volume170
Issue number1-2
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Carbon nanofibers
Ointments
Electrodes
Paraffin waxes
Graphite
Composite materials
Voltammetry
Chemical analysis
Electrochemical impedance spectroscopy
Electrochemical properties
Ammonium Compounds
Cyclic voltammetry
Cations
Buffers
Nucleation
Metals
Ions
Adsorption
Substrates

Keywords

  • solidified paste electrode
  • DNA biosensor
  • nucleation stripping voltammetry
  • carbon nanofibers
  • nickel-DNA interaction

Cite this

dsDNA modified carbon nanofiber-solidified paste electrodes: probing Ni(II)-dsDNA interactions. / Ferancova, A; Rassaei, Liza; Marken, Frank; Labuda, J; Sillanpaa, M.

In: Microchimica Acta, Vol. 170, No. 1-2, 08.2010, p. 155-164.

Research output: Contribution to journalArticle

Ferancova, A ; Rassaei, Liza ; Marken, Frank ; Labuda, J ; Sillanpaa, M. / dsDNA modified carbon nanofiber-solidified paste electrodes: probing Ni(II)-dsDNA interactions. In: Microchimica Acta. 2010 ; Vol. 170, No. 1-2. pp. 155-164.
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