dsDNA modified carbon nanofiber-solidified paste electrodes: probing Ni(II)-dsDNA interactions

A Ferancova, Liza Rassaei, Frank Marken, J Labuda, M Sillanpaa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to develop a renewable electrode surface, carbon nanofibers (CNF) were embedded into solidified paste electrodes using a composite of paraffin wax and paraffin oil. A range of different compositions was surveyed and the optimal composition of the paste for electroanalysis was found to be 43% of CNF, 41% of paraffin wax, and 16% of paraffin oil. The electrochemical properties of the novel composite electrode were investigated using cyclic voltammetry and electrochemical impedance spectroscopy and compared to those of similar graphite-solidified paste electrodes. The carbon nanofibers enhance the activity of the surface of the electrode and provide a good substrate for the adsorption and voltammetric detection of dsDNA. Responses of dsDNA bases and Ni2+ ions accumulated from ammonium buffer pH 8.5 (with a Langmuirian binding constant of 10(5) mol(-1) L) were investigated and a limit of detection of 7 nmol L-1 (at 3 sigma) was obtained using "nucleation stripping voltammetry". Interferences by other metal cations are examined and discussed.
Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalMicrochimica Acta
Volume170
Issue number1-2
DOIs
Publication statusPublished - Aug 2010

Keywords

  • solidified paste electrode
  • DNA biosensor
  • nucleation stripping voltammetry
  • carbon nanofibers
  • nickel-DNA interaction

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