Domestication of Campylobacter jejuni NCTC 11168

Ben Pascoe, Lisa K Williams, Jessica K Calland, Guillaume Meric, Matthew D Hitchings, Myles Dyer, Joseph Ryder, Sophie Shaw, Bruno S Lopes, Cosmin Chintoan-Uta, Elaine Allan, Ana Vidal, Catherine Fearnley, Paul Everest, Justin A Pachebat, Tristan A Cogan, Mark P Stevens, Thomas J Humphrey, Thomas S Wilkinson, Alison J Cody & 12 others Frances M Colles, Keith A Jolley, Martin C J Maiden, Norval Strachan, Bruce M Pearson, Dennis Linton, Brendan W Wren, Julian Parkhill, David J Kelly, Arnoud H M van Vliet, Ken J Forbes, Samuel K Sheppard

Research output: Contribution to journalArticle

Abstract

Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

Original languageEnglish
JournalMicrobial Genomics
Early online date16 Jul 2019
DOIs
Publication statusPublished - 16 Jul 2019

Cite this

Domestication of Campylobacter jejuni NCTC 11168. / Pascoe, Ben; Williams, Lisa K; Calland, Jessica K; Meric, Guillaume; Hitchings, Matthew D; Dyer, Myles; Ryder, Joseph; Shaw, Sophie; Lopes, Bruno S; Chintoan-Uta, Cosmin; Allan, Elaine; Vidal, Ana; Fearnley, Catherine; Everest, Paul; Pachebat, Justin A; Cogan, Tristan A; Stevens, Mark P; Humphrey, Thomas J; Wilkinson, Thomas S; Cody, Alison J; Colles, Frances M; Jolley, Keith A; Maiden, Martin C J; Strachan, Norval; Pearson, Bruce M; Linton, Dennis; Wren, Brendan W; Parkhill, Julian; Kelly, David J; van Vliet, Arnoud H M; Forbes, Ken J; Sheppard, Samuel K.

In: Microbial Genomics, 16.07.2019.

Research output: Contribution to journalArticle

Pascoe, B, Williams, LK, Calland, JK, Meric, G, Hitchings, MD, Dyer, M, Ryder, J, Shaw, S, Lopes, BS, Chintoan-Uta, C, Allan, E, Vidal, A, Fearnley, C, Everest, P, Pachebat, JA, Cogan, TA, Stevens, MP, Humphrey, TJ, Wilkinson, TS, Cody, AJ, Colles, FM, Jolley, KA, Maiden, MCJ, Strachan, N, Pearson, BM, Linton, D, Wren, BW, Parkhill, J, Kelly, DJ, van Vliet, AHM, Forbes, KJ & Sheppard, SK 2019, 'Domestication of Campylobacter jejuni NCTC 11168', Microbial Genomics. https://doi.org/10.1099/mgen.0.000279
Pascoe, Ben ; Williams, Lisa K ; Calland, Jessica K ; Meric, Guillaume ; Hitchings, Matthew D ; Dyer, Myles ; Ryder, Joseph ; Shaw, Sophie ; Lopes, Bruno S ; Chintoan-Uta, Cosmin ; Allan, Elaine ; Vidal, Ana ; Fearnley, Catherine ; Everest, Paul ; Pachebat, Justin A ; Cogan, Tristan A ; Stevens, Mark P ; Humphrey, Thomas J ; Wilkinson, Thomas S ; Cody, Alison J ; Colles, Frances M ; Jolley, Keith A ; Maiden, Martin C J ; Strachan, Norval ; Pearson, Bruce M ; Linton, Dennis ; Wren, Brendan W ; Parkhill, Julian ; Kelly, David J ; van Vliet, Arnoud H M ; Forbes, Ken J ; Sheppard, Samuel K. / Domestication of Campylobacter jejuni NCTC 11168. In: Microbial Genomics. 2019.
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AU - Williams, Lisa K

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AU - Dyer, Myles

AU - Ryder, Joseph

AU - Shaw, Sophie

AU - Lopes, Bruno S

AU - Chintoan-Uta, Cosmin

AU - Allan, Elaine

AU - Vidal, Ana

AU - Fearnley, Catherine

AU - Everest, Paul

AU - Pachebat, Justin A

AU - Cogan, Tristan A

AU - Stevens, Mark P

AU - Humphrey, Thomas J

AU - Wilkinson, Thomas S

AU - Cody, Alison J

AU - Colles, Frances M

AU - Jolley, Keith A

AU - Maiden, Martin C J

AU - Strachan, Norval

AU - Pearson, Bruce M

AU - Linton, Dennis

AU - Wren, Brendan W

AU - Parkhill, Julian

AU - Kelly, David J

AU - van Vliet, Arnoud H M

AU - Forbes, Ken J

AU - Sheppard, Samuel K

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N2 - Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

AB - Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

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