DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry

Yoshinori Okamoto, Naoko Yoshida, Toru Suzuki, Nobuhiro Shimozawa, Maki Asami, Tomonari Matsuda, Nakao Kojima, Anthony C. F. Perry, Tatsuyuki Takada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Following fertilization in mammals, paternal genomic 5-methyl-2′-deoxycytidine (5 mC) content is thought to decrease via oxidation to 5-hydroxymethyl-2′-deoxycytidine (5 hmC). This reciprocal model of demethylation and hydroxymethylation is inferred from indirect, non-quantitative methods. We here report direct quantification of genomic 5 mC and 5 hmC in mouse embryos by small scale liquid chromatographic tandem mass spectrometry (SMM). Profiles of absolute 5 mC levels in embryos produced by in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) were almost identical. By 10 h after fertilization, 5 mC levels had declined by ~40%, consistent with active genomic DNA demethylation. Levels of 5 mC in androgenotes (containing only a paternal genome) and parthenogenotes (containing only a maternal genome) underwent active 5 mC loss in the first 6 h, showing that both parental genomes can undergo demethylation independently. We found no evidence for net loss of 5 mC 10–48 h after fertilization, implying that any passive ‘demethylation’ following DNA replication was balanced by active 5 mC maintenance methylation. However, levels of 5 mC declined during development after 48 h, to 1% (measured as a fraction of G-residues) in blastocysts (~96 h). 5 hmC levels were consistently low (<0.2% of G-residues) throughout development in normal diploid embryos. This work directly quantifies the dynamics of global genomic DNA modification in mouse preimplantation embryos, suggesting that SMM will be applicable to other biomedical situations with limiting sample sizes.
LanguageEnglish
Article number19134
JournalScientific Reports
Volume6
DOIs
StatusPublished - 11 Jan 2016

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5-methyldeoxycytidine
Blastocyst
DNA Methylation
Mass Spectrometry
Fertilization
Embryonic Structures
Genome
Intracytoplasmic Sperm Injections
DNA

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DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry. / Okamoto, Yoshinori ; Yoshida, Naoko; Suzuki, Toru; Shimozawa, Nobuhiro ; Asami, Maki; Matsuda, Tomonari ; Kojima, Nakao ; Perry, Anthony C. F.; Takada, Tatsuyuki .

In: Scientific Reports, Vol. 6, 19134, 11.01.2016.

Research output: Contribution to journalArticle

Okamoto, Yoshinori ; Yoshida, Naoko ; Suzuki, Toru ; Shimozawa, Nobuhiro ; Asami, Maki ; Matsuda, Tomonari ; Kojima, Nakao ; Perry, Anthony C. F. ; Takada, Tatsuyuki . / DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry. In: Scientific Reports. 2016 ; Vol. 6.
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