Diversity of microbes associated with the marine sponge, Haliclona simulans, isolated from Irish waters and identification of polyketide synthase genes from the sponge metagenome

Jonathan Kennedy, Caroline E Codling, Brian V Jones, Alan D W Dobson, Julian R Marchesi

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Samples of the sponge Haliclona simulans were collected from Irish waters and subjected to a culture-independent analysis to determine the microbial, polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) diversity. 16S rRNA gene libraries were prepared from total sponge, bacterial enriched sponge and seawater samples. Eight phyla from the Bacteria were detected in the sponge by phylogenetic analyses of the 16S rRNA gene libraries. The most abundant phylum in the total sponge library was the Proteobacteria (86%), with the majority of these clones being from the gamma-Proteobacteria (77%); two groups of clones were dominant and together made up 69% of the total. Both of these groups were related to other sponge-derived microbes and comprised novel genera. Within the other bacterial phyla groups of clones representing novel candidate genera within the phyla Verrucomicrobia and Lentisphaerae were also found. Selective enrichment of the bacterial component of the sponge prior to 16S rRNA gene analysis resulted in a 16S rRNA gene library dominated by a novel genus of delta-Proteobacteria, most closely related to the Bdellovibrio. The potential for the sponge microbiota to produce secondary metabolites was also analysed by polymerase chain reaction amplification of PKS and NRPS genes. While no NRPS sequences were isolated seven ketosynthase (KS) sequences were obtained from the sponge metagenome. Analyses of these clones revealed a diverse collection of PKS sequences which were most closely affiliated with PKS from members of the Cyanobacteria, Myxobacteria and Dinoflagellata.

Original languageEnglish
Pages (from-to)1888-1902
Number of pages15
JournalEnvironmental Microbiology
Volume10
Issue number7
Early online date21 Apr 2008
DOIs
Publication statusPublished - 1 Jul 2008

Keywords

  • Animals
  • Biodiversity
  • DNA, Bacterial/analysis
  • DNA, Ribosomal/analysis
  • Gammaproteobacteria/enzymology
  • Genomic Library
  • Haliclona/microbiology
  • Molecular Sequence Data
  • Peptide Synthases/metabolism
  • Polyketide Synthases/genetics
  • RNA, Bacterial/genetics
  • RNA, Ribosomal, 16S/genetics
  • Seawater

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