Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells

James A. Heward; Benoit T. Roux; Mark A. Lindsay

doi:10.1016/j.febslet.2014.12.026

Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells

James A. Heward, Benoit T. Roux, Mark A. Lindsay

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Abstract

Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression. In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS). Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.

Original language	English
Pages (from-to)	396-406
Number of pages	11
Journal	FEBS Letters
Volume	589
Issue number	3
Early online date	29 Dec 2014
DOIs	https://doi.org/10.1016/j.febslet.2014.12.026
Publication status	Published - 30 Jan 2015

Keywords

Enhancer RNA
ERK and p38
Lipopolysaccharide
Long non-coding RNA
Monocyte
NF-κB

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10.1016/j.febslet.2014.12.026

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title = "Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells",

abstract = "Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression. In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS). Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.",

keywords = "Enhancer RNA, ERK and p38, Lipopolysaccharide, Long non-coding RNA, Monocyte, NF-κB",

author = "Heward, {James A.} and Roux, {Benoit T.} and Lindsay, {Mark A.}",

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AU - Heward, James A.

AU - Roux, Benoit T.

AU - Lindsay, Mark A.

PY - 2015/1/30

Y1 - 2015/1/30

N2 - Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression. In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS). Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.

AB - Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression. In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS). Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.

KW - Enhancer RNA

KW - ERK and p38

KW - Lipopolysaccharide

KW - Long non-coding RNA

KW - Monocyte

KW - NF-κB

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DO - 10.1016/j.febslet.2014.12.026

M3 - Article

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -

Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells

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Keywords

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Are Long Intergenic Non-ccoding RNAs Central Regulators of Inflammation and the Innate Immune Response?

Mark Lindsay

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Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells

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Are Long Intergenic Non-ccoding RNAs Central Regulators of Inflammation and the Innate Immune Response?

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Mark Lindsay

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