Divergent intracellular pathways regulate interleukin-1β-induced miR-146a and miR-146b expression and chemokine release in human alveolar epithelial cells

Mark M Perry, Andrew E Williams, Eleni Tsitsiou, Hanna M Larner-Svensson, Mark A Lindsay

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

We have previously reported that IL-β-induced miR-146a and miR-146b expression negatively regulates IL-8 and RANTES release in human alveolar A549 epithelial cells. To determine the intracellular pathways that regulate this response, we demonstrate IL-1β-induced activation of the nuclear factor (NF)-κB, extracellular regulated kinase (ERK)-1/2, c-jun N-terminal kinase (JNK)-1/2 and p38 mitogen activated kinase (MAP) kinase pathways. Subsequent pharmacological studies show that IL-1β-induced miR-146a, IL-8 and RANTES production was regulated via NF-κB and JNK-1/2 whilst miR-146b expression was mediated via MEK-1/2 and JNK-1/2. These divergent intracellular pathways likely explain the differential expression and biological action of the miR-146 isoforms.
Original languageEnglish
Pages (from-to)3349-3355
Number of pages7
JournalFEBS Letters
Volume583
Issue number20
Early online date25 Sep 2009
DOIs
Publication statusPublished - 20 Oct 2009

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