Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogaster

W Wood, C Faria, A Jacinto

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Drosophila melanogaster hemocytes are highly motile macrophage-like cells that undergo a stereotypic pattern of migration to populate the whole embryo by late embryogenesis. We demonstrate that the migratory patterns of hemocytes at the embryonic ventral midline are orchestrated by chemotactic signals from the PDGF/VEGF ligands Pvf2 and -3 and that these directed migrations occur independently of phosphoinositide 3-kinase (PI3K) signaling. In contrast, using both laser ablation and a novel wounding assay that allows localized treatment inhibitory drugs, we show that PI3K is essential for hemocyte chemotaxis toward wounds and that Pvf signals and PDGF/VEGF receptor expression are not required for this rapid chemotactic response. Our results demonstrate that at least two separate mechanisms operate in D. melanogaster embryos to direct hemocyte migration and show that although PI3K is crucial for hemocytes to sense a chemotactic gradient from a wound, it is not required to sense the growth factor signals that coordinate their developmental migrations along the ventral midline during embryogenesis.
Original languageEnglish
Pages (from-to)405-416
Number of pages12
JournalThe Journal of Cell Biology (JCB)
Volume173
Issue number3
DOIs
Publication statusPublished - 2006

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Hemocytes
Chemotaxis
Drosophila melanogaster
Wound Healing
1-Phosphatidylinositol 4-Kinase
Embryonic Development
Embryonic Structures
Platelet-Derived Growth Factor Receptors
Vascular Endothelial Growth Factor Receptor
Wounds and Injuries
Laser Therapy
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Macrophages
Ligands
Pharmaceutical Preparations

Cite this

Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogaster. / Wood, W; Faria, C; Jacinto, A.

In: The Journal of Cell Biology (JCB), Vol. 173, No. 3, 2006, p. 405-416.

Research output: Contribution to journalArticle

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AB - Drosophila melanogaster hemocytes are highly motile macrophage-like cells that undergo a stereotypic pattern of migration to populate the whole embryo by late embryogenesis. We demonstrate that the migratory patterns of hemocytes at the embryonic ventral midline are orchestrated by chemotactic signals from the PDGF/VEGF ligands Pvf2 and -3 and that these directed migrations occur independently of phosphoinositide 3-kinase (PI3K) signaling. In contrast, using both laser ablation and a novel wounding assay that allows localized treatment inhibitory drugs, we show that PI3K is essential for hemocyte chemotaxis toward wounds and that Pvf signals and PDGF/VEGF receptor expression are not required for this rapid chemotactic response. Our results demonstrate that at least two separate mechanisms operate in D. melanogaster embryos to direct hemocyte migration and show that although PI3K is crucial for hemocytes to sense a chemotactic gradient from a wound, it is not required to sense the growth factor signals that coordinate their developmental migrations along the ventral midline during embryogenesis.

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