Disproportionate growth in mice with Igf-2 transgenes

A Ward, P Bates, Rebecca Fisher, L Richardson, C F Graham

Research output: Contribution to journalArticlepeer-review

Abstract

Injection transgenesis was used to study the long-term effects of excess insulin-like growth factor II on mouse growth and differentiation. By using a construct in which the coding region of the mouse insulin like growth factor II gene (Igf-2) was placed under the control of a keratin gene promoter, four transgenic lines were established, all of which displayed overgrowth of the skin as judged by wrinkling. In addition to high levels of expression in the skin, transgene transcripts were also present in the alimentary canal and uterus. At most of the sites of transgene expression the cell number (DNA content) was greatly increased, indicating a local action of the excess insulin-like growth factor II on cell multiplication. Adult total live weight was slightly increased and there was no macroscopic evidence of tumor formation. The characteristics of these transgenic mice indicate distinct local and systemic actions for insulin-like growth factor II.

Original languageEnglish
Pages (from-to)10365-9
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number22
Publication statusPublished - 25 Oct 1994

Keywords

  • Aging
  • Animals
  • Cattle
  • Cell Division
  • DNA
  • Female
  • Hyperplasia
  • Insulin-Like Growth Factor II
  • Keratins
  • Male
  • Mice
  • Mice, Transgenic
  • Organ Specificity
  • Promoter Regions, Genetic
  • Skin Aging
  • Transcription, Genetic
  • Journal Article

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