Diol appended quenchers for fluorescein boronic acid

S A Elfeky, S E Flower, N Masumoto, F D'Hooge, L Labarthe, W B Chen, C Len, T D James, J S Fossey

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
Original languageEnglish
Pages (from-to)581-588
Number of pages8
JournalChemistry - An Asian Journal
Volume5
Issue number3
DOIs
Publication statusPublished - Feb 2010

Fingerprint Dive into the research topics of 'Diol appended quenchers for fluorescein boronic acid'. Together they form a unique fingerprint.

  • Cite this

    Elfeky, S. A., Flower, S. E., Masumoto, N., D'Hooge, F., Labarthe, L., Chen, W. B., Len, C., James, T. D., & Fossey, J. S. (2010). Diol appended quenchers for fluorescein boronic acid. Chemistry - An Asian Journal, 5(3), 581-588. https://doi.org/10.1002/asia.200900386