Diol appended quenchers for fluorescein boronic acid

S A Elfeky; S E Flower; N Masumoto; F D'Hooge; L Labarthe; W B Chen; C Len; T D James; J S Fossey

doi:10.1002/asia.200900386

Diol appended quenchers for fluorescein boronic acid

S A Elfeky, S E Flower, N Masumoto, F D'Hooge, L Labarthe, W B Chen, C Len, T D James, J S Fossey

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

28 Citations (SciVal)

Abstract

Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

Original language	English
Pages (from-to)	581-588
Number of pages	8
Journal	Chemistry - An Asian Journal
Volume	5
Issue number	3
DOIs	https://doi.org/10.1002/asia.200900386
Publication status	Published - Feb 2010

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abstract = "Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.",

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T1 - Diol appended quenchers for fluorescein boronic acid

AU - Elfeky, S A

AU - Flower, S E

AU - Masumoto, N

AU - D'Hooge, F

AU - Labarthe, L

AU - Chen, W B

AU - Len, C

AU - James, T D

AU - Fossey, J S

PY - 2010/2

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AB - Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

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JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 3

ER -

Diol appended quenchers for fluorescein boronic acid

Abstract

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