Disclosed are methods, kits and cells for screening for an inhibitor of association between candidate binding partners, such as for screening for antagonists of amyloid peptides. The methods, kits and cells employ a reporter expression cassette and hybrid proteins. The reporter expression cassette encodes a reporter and comprises at least one DNA-binding site. Each hybrid protein comprises a candidate binding partner and a component of a DNA binding protein and, upon association, form a DNA-binding complex capable of binding to the at least one binding site and inhibiting expression of the reporter. The methods, kits and cells find application, for example, in the identification of inhibitors that may be useful in treating diseases associated with protein aggregation, such as Alzheimer's Disease and Parkinson's Disease.

Original languageEnglish
Patent numberWO2021156316
Priority date4/02/20
Publication statusPublished - 12 Aug 2021


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