Differentiated melanocyte cell division occurs in vivo and is promoted by mutations in Mitf

Kerrie L. Taylor, James A. Lister, Zhiqiang Q. Zeng, Hironori Ishizaki, C Anderson, Robert N. Kelsh, Ian J. Jackson, E. Elizabeth Patton

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Coordination of cell proliferation and differentiation is crucial for tissue formation, repair and regeneration. Some tissues, such as skin and blood, depend on differentiation of a pluripotent stem cell population, whereas others depend on the division of differentiated cells. In development and in the hair follicle, pigmented melanocytes are derived from undifferentiated precursor cells or stem cells. However, differentiated melanocytes may also have proliferative capacity in animals, and the potential for differentiated melanocyte cell division in development and regeneration remains largely unexplored. Here, we use time-lapse imaging of the developing zebrafish to show that while most melanocytes arise from undifferentiated precursor cells, an unexpected subpopulation of differentiated melanocytes arises by cell division. Depletion of the overall melanocyte population triggers a regeneration phase in which differentiated melanocyte division is significantly enhanced, particularly in young differentiated melanocytes. Additionally, we find reduced levels of Mitf activity using an mitfa temperature-sensitive line results in a dramatic increase in differentiated melanocyte cell division. This supports models that in addition to promoting differentiation, Mitf also promotes withdrawal from the cell cycle. We suggest differentiated cell division is relevant to melanoma progression because the human melanoma mutation MITF(4T Delta 2B) promotes increased and serial differentiated melanocyte division in zebrafish. These results reveal a novel pathway of differentiated melanocyte division in vivo, and that Mitf activity is essential for maintaining cell cycle arrest in differentiated melanocytes.
Original languageEnglish
Pages (from-to)3579-3589
JournalDevelopment
Volume138
Issue number16
DOIs
Publication statusPublished - 2011

Fingerprint

Melanocytes
Cell Division
Mutation
Regeneration
Zebrafish
Melanoma
Time-Lapse Imaging
Pluripotent Stem Cells
Hair Follicle
Cell Cycle Checkpoints
Population
Cell Differentiation
Cell Cycle
Stem Cells
Cell Proliferation

Keywords

  • melanocyte
  • division
  • regeneration
  • cell division
  • zebrafish
  • imaging

Cite this

Taylor, K. L., Lister, J. A., Zeng, Z. Q., Ishizaki, H., Anderson, C., Kelsh, R. N., ... Patton, E. E. (2011). Differentiated melanocyte cell division occurs in vivo and is promoted by mutations in Mitf. Development, 138(16), 3579-3589. https://doi.org/10.1242/dev.064014

Differentiated melanocyte cell division occurs in vivo and is promoted by mutations in Mitf. / Taylor, Kerrie L.; Lister, James A.; Zeng, Zhiqiang Q.; Ishizaki, Hironori; Anderson, C; Kelsh, Robert N.; Jackson, Ian J.; Patton, E. Elizabeth.

In: Development, Vol. 138, No. 16, 2011, p. 3579-3589.

Research output: Contribution to journalArticle

Taylor, KL, Lister, JA, Zeng, ZQ, Ishizaki, H, Anderson, C, Kelsh, RN, Jackson, IJ & Patton, EE 2011, 'Differentiated melanocyte cell division occurs in vivo and is promoted by mutations in Mitf', Development, vol. 138, no. 16, pp. 3579-3589. https://doi.org/10.1242/dev.064014
Taylor, Kerrie L. ; Lister, James A. ; Zeng, Zhiqiang Q. ; Ishizaki, Hironori ; Anderson, C ; Kelsh, Robert N. ; Jackson, Ian J. ; Patton, E. Elizabeth. / Differentiated melanocyte cell division occurs in vivo and is promoted by mutations in Mitf. In: Development. 2011 ; Vol. 138, No. 16. pp. 3579-3589.
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