TY - JOUR
T1 - Differential regulation of polysome mRNA levels in mouse Hepa-1C1C7 cells exposed to dioxin
AU - Thornley, Jessica A.
AU - Trask, Heidi W.
AU - Ridley, Christian J. A.
AU - Korc, Murray
AU - Gui, Jiang
AU - Ringelberg, Carol S.
AU - Wang, Sinny
AU - Tomlinson, Craig R.
PY - 2011/10
Y1 - 2011/10
N2 - The environmental agent 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or dioxin) causes a multitude of human illnesses. In order to more fully understand the underlying biology of TCDD toxicity, we tested the hypothesis that new candidate genes could be identified using polysome RNA from TCDD-treated mouse Hepa-1c1c7 cells. We found that (i) differentially expressed whole cell and cytoplasm RNA levels are both poor predictors of polysome RNA levels; (ii) for a majority of RNAs, differential RNA levels are regulated independently in the nucleus, cytoplasm, and polysomes; (iii) for the remaining polysome RNAs, levels are regulated via several different mechanisms, including a "tagging" of mRNAs in the nucleus for immediate polysome entry; and (iv) most importantly, a gene list derived from differentially expressed polysome RNA generated new genes and cell pathways potentially related to TCDD biology.
AB - The environmental agent 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or dioxin) causes a multitude of human illnesses. In order to more fully understand the underlying biology of TCDD toxicity, we tested the hypothesis that new candidate genes could be identified using polysome RNA from TCDD-treated mouse Hepa-1c1c7 cells. We found that (i) differentially expressed whole cell and cytoplasm RNA levels are both poor predictors of polysome RNA levels; (ii) for a majority of RNAs, differential RNA levels are regulated independently in the nucleus, cytoplasm, and polysomes; (iii) for the remaining polysome RNAs, levels are regulated via several different mechanisms, including a "tagging" of mRNAs in the nucleus for immediate polysome entry; and (iv) most importantly, a gene list derived from differentially expressed polysome RNA generated new genes and cell pathways potentially related to TCDD biology.
UR - http://www.scopus.com/inward/record.url?scp=84860419199&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.tiv.2011.04.020
U2 - 10.1016/j.tiv.2011.04.020
DO - 10.1016/j.tiv.2011.04.020
M3 - Article
SN - 0887-2333
VL - 25
SP - 1457
EP - 1467
JO - Toxicology in Vitro
JF - Toxicology in Vitro
IS - 7
ER -