Differential isotype recognition of two centromere associated polypeptides by immunoblotting in connective tissue disease

N J McHugh; I E James; P J Maddison

Differential isotype recognition of two centromere associated polypeptides by immunoblotting in connective tissue disease

N J McHugh, I E James, P J Maddison

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

On investigating the immunoblotting profile of 65 systemic sclerosis patients, a 140 kD polypeptide was recognised by sera from 16, when immunoblotted against a nuclear-enriched K562 cell sonicate. All 16 sera contained anticentromere antibodies (ACA) detected by immunofluorescence (IF) and 15 of 16 also recognized a 19 kD polypeptide on immunoblotting. Two ACA positive sera failed to recognize the 140 kD polypeptide but one of these recognized the 19 kD polypeptide. The 140 kD polypeptide identified a group with more limited skin involvement (P less than 0.05) and all 16 had Raynaud's phenomenon. The sera from three of 100 systemic lupus erythematosus (SLE) patients also recognized both polypeptides. On investigating the isotype specificity, the 140 kD polypeptide was strongly detected by an IgM autoantibody and the 19 kD polypeptide by an IgG autoantibody.

Original language	English
Pages (from-to)	457-64
Number of pages	8
Journal	Clinical And Experimental Immunology
Volume	72
Issue number	3
Publication status	Published - Jun 1988

Keywords

Antibodies, Antinuclear
Centromere
Chromosomes
Electrophoresis, Polyacrylamide Gel
England
Felty Syndrome
Fluorescent Antibody Technique
Humans
Immunoglobulin Isotypes
Longitudinal Studies
Lupus Erythematosus, Systemic
Peptides
Scleroderma, Systemic

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title = "Differential isotype recognition of two centromere associated polypeptides by immunoblotting in connective tissue disease",

abstract = "On investigating the immunoblotting profile of 65 systemic sclerosis patients, a 140 kD polypeptide was recognised by sera from 16, when immunoblotted against a nuclear-enriched K562 cell sonicate. All 16 sera contained anticentromere antibodies (ACA) detected by immunofluorescence (IF) and 15 of 16 also recognized a 19 kD polypeptide on immunoblotting. Two ACA positive sera failed to recognize the 140 kD polypeptide but one of these recognized the 19 kD polypeptide. The 140 kD polypeptide identified a group with more limited skin involvement (P less than 0.05) and all 16 had Raynaud's phenomenon. The sera from three of 100 systemic lupus erythematosus (SLE) patients also recognized both polypeptides. On investigating the isotype specificity, the 140 kD polypeptide was strongly detected by an IgM autoantibody and the 19 kD polypeptide by an IgG autoantibody.",

keywords = "Antibodies, Antinuclear, Centromere, Chromosomes, Electrophoresis, Polyacrylamide Gel, England, Felty Syndrome, Fluorescent Antibody Technique, Humans, Immunoglobulin Isotypes, Longitudinal Studies, Lupus Erythematosus, Systemic, Peptides, Scleroderma, Systemic",

author = "McHugh, {N J} and James, {I E} and Maddison, {P J}",

year = "1988",

month = jun,

language = "English",

volume = "72",

pages = "457--64",

journal = "Clinical And Experimental Immunology",

issn = "0009-9104",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Differential isotype recognition of two centromere associated polypeptides by immunoblotting in connective tissue disease

AU - McHugh, N J

AU - James, I E

AU - Maddison, P J

PY - 1988/6

Y1 - 1988/6

N2 - On investigating the immunoblotting profile of 65 systemic sclerosis patients, a 140 kD polypeptide was recognised by sera from 16, when immunoblotted against a nuclear-enriched K562 cell sonicate. All 16 sera contained anticentromere antibodies (ACA) detected by immunofluorescence (IF) and 15 of 16 also recognized a 19 kD polypeptide on immunoblotting. Two ACA positive sera failed to recognize the 140 kD polypeptide but one of these recognized the 19 kD polypeptide. The 140 kD polypeptide identified a group with more limited skin involvement (P less than 0.05) and all 16 had Raynaud's phenomenon. The sera from three of 100 systemic lupus erythematosus (SLE) patients also recognized both polypeptides. On investigating the isotype specificity, the 140 kD polypeptide was strongly detected by an IgM autoantibody and the 19 kD polypeptide by an IgG autoantibody.

AB - On investigating the immunoblotting profile of 65 systemic sclerosis patients, a 140 kD polypeptide was recognised by sera from 16, when immunoblotted against a nuclear-enriched K562 cell sonicate. All 16 sera contained anticentromere antibodies (ACA) detected by immunofluorescence (IF) and 15 of 16 also recognized a 19 kD polypeptide on immunoblotting. Two ACA positive sera failed to recognize the 140 kD polypeptide but one of these recognized the 19 kD polypeptide. The 140 kD polypeptide identified a group with more limited skin involvement (P less than 0.05) and all 16 had Raynaud's phenomenon. The sera from three of 100 systemic lupus erythematosus (SLE) patients also recognized both polypeptides. On investigating the isotype specificity, the 140 kD polypeptide was strongly detected by an IgM autoantibody and the 19 kD polypeptide by an IgG autoantibody.

KW - Antibodies, Antinuclear

KW - Centromere

KW - Chromosomes

KW - Electrophoresis, Polyacrylamide Gel

KW - England

KW - Felty Syndrome

KW - Fluorescent Antibody Technique

KW - Humans

KW - Immunoglobulin Isotypes

KW - Longitudinal Studies

KW - Lupus Erythematosus, Systemic

KW - Peptides

KW - Scleroderma, Systemic

M3 - Article

C2 - 3048806

SN - 0009-9104

VL - 72

SP - 457

EP - 464

JO - Clinical And Experimental Immunology

JF - Clinical And Experimental Immunology

IS - 3

ER -

Differential isotype recognition of two centromere associated polypeptides by immunoblotting in connective tissue disease

Abstract

Keywords

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