Differential coupling of alpha 7 and non-alpha 7 nicotinic acetylcholine receptors to calcium-induced calcium release and voltage-operated calcium channels in PC12 cells

J A Dickinson, K E Hanrott, M H S Mok, J N C Kew, S Wonnacott

Research output: Contribution to journalArticle

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Abstract

Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated cation channels that can modulate various neuronal processes by altering intracellular Ca2+ levels. Following nAChR stimulation Ca2+ can enter cells either directly, through the intrinsic ion channel, or indirectly following voltage-operated Ca2+ channel (VOCC) activation; Ca2+ levels can subsequently be amplified via Ca2+-induced Ca2+ release from intracellular stores. We have used subtype-selective nAChR agonists to investigate the Ca2+ sources contributing to alpha 7 and non-alpha 7 nAChR-mediated increases in intracellular Ca2+ in PC12 cells. Application of the alpha 7 nAChR positive allosteric modulator PNU 120596 (10 mu M), in conjunction with the alpha 7 nAChR agonist, compound A [(R)-N-(1-azabicyclo [2.2.2]oct-3-yl)(5-(2-pyridyl)thiophene-2-carboxamide), 10 nM], produces a rapid increase in fluo-3 fluorescence that is prevented by the selective alpha 7 nAChR antagonist alpha-bungarotoxin. The non-alpha 7 nAChR agonist 5-Iodo-A-85380 produces alpha-bungarotoxin-insensitive increases in intracellular Ca2+ (EC50 11.2 mu M). Using these selective agonists or KCl in conjunction with general and selective VOCC inhibitors, we demonstrate that the primary route of Ca2+ entry following either non-alpha 7 nAChR activation or KCl stimulation is via L-type VOCCs. In contrast, the alpha 7 nAChR-mediated response is unaffected by VOCC blockers but is inhibited by modulators of intracellular Ca2+ stores. These results indicate that alpha 7 and non-alpha 7 nAChRs are differentially coupled to Ca2+-induced Ca2+ release and VOCCs, respectively.
LanguageEnglish
Pages1089-1096
Number of pages8
JournalJournal of Neurochemistry
Volume100
Issue number4
DOIs
StatusPublished - Feb 2007

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Bungarotoxins
PC12 Cells
Nicotinic Receptors
Calcium Channels
A 85380
Calcium
Ligand-Gated Ion Channels
Modulators
Electric potential
Chemical activation
Ion Channels
Cations
Fluorescence
Ligands
N-(1-azabicyclo(2.2.2)oct-3-yl)(5-(2-pyridyl)thiophene-2-carboxamide)
1-(5-chloro-2,4-dimethoxyphenyl)-3-(5-methylisoxazol-3-yl)urea
Fluo-3

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Differential coupling of alpha 7 and non-alpha 7 nicotinic acetylcholine receptors to calcium-induced calcium release and voltage-operated calcium channels in PC12 cells. / Dickinson, J A; Hanrott, K E; Mok, M H S; Kew, J N C; Wonnacott, S.

In: Journal of Neurochemistry, Vol. 100, No. 4, 02.2007, p. 1089-1096.

Research output: Contribution to journalArticle

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T1 - Differential coupling of alpha 7 and non-alpha 7 nicotinic acetylcholine receptors to calcium-induced calcium release and voltage-operated calcium channels in PC12 cells

AU - Dickinson,J A

AU - Hanrott,K E

AU - Mok,M H S

AU - Kew,J N C

AU - Wonnacott,S

PY - 2007/2

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N2 - Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated cation channels that can modulate various neuronal processes by altering intracellular Ca2+ levels. Following nAChR stimulation Ca2+ can enter cells either directly, through the intrinsic ion channel, or indirectly following voltage-operated Ca2+ channel (VOCC) activation; Ca2+ levels can subsequently be amplified via Ca2+-induced Ca2+ release from intracellular stores. We have used subtype-selective nAChR agonists to investigate the Ca2+ sources contributing to alpha 7 and non-alpha 7 nAChR-mediated increases in intracellular Ca2+ in PC12 cells. Application of the alpha 7 nAChR positive allosteric modulator PNU 120596 (10 mu M), in conjunction with the alpha 7 nAChR agonist, compound A [(R)-N-(1-azabicyclo [2.2.2]oct-3-yl)(5-(2-pyridyl)thiophene-2-carboxamide), 10 nM], produces a rapid increase in fluo-3 fluorescence that is prevented by the selective alpha 7 nAChR antagonist alpha-bungarotoxin. The non-alpha 7 nAChR agonist 5-Iodo-A-85380 produces alpha-bungarotoxin-insensitive increases in intracellular Ca2+ (EC50 11.2 mu M). Using these selective agonists or KCl in conjunction with general and selective VOCC inhibitors, we demonstrate that the primary route of Ca2+ entry following either non-alpha 7 nAChR activation or KCl stimulation is via L-type VOCCs. In contrast, the alpha 7 nAChR-mediated response is unaffected by VOCC blockers but is inhibited by modulators of intracellular Ca2+ stores. These results indicate that alpha 7 and non-alpha 7 nAChRs are differentially coupled to Ca2+-induced Ca2+ release and VOCCs, respectively.

AB - Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated cation channels that can modulate various neuronal processes by altering intracellular Ca2+ levels. Following nAChR stimulation Ca2+ can enter cells either directly, through the intrinsic ion channel, or indirectly following voltage-operated Ca2+ channel (VOCC) activation; Ca2+ levels can subsequently be amplified via Ca2+-induced Ca2+ release from intracellular stores. We have used subtype-selective nAChR agonists to investigate the Ca2+ sources contributing to alpha 7 and non-alpha 7 nAChR-mediated increases in intracellular Ca2+ in PC12 cells. Application of the alpha 7 nAChR positive allosteric modulator PNU 120596 (10 mu M), in conjunction with the alpha 7 nAChR agonist, compound A [(R)-N-(1-azabicyclo [2.2.2]oct-3-yl)(5-(2-pyridyl)thiophene-2-carboxamide), 10 nM], produces a rapid increase in fluo-3 fluorescence that is prevented by the selective alpha 7 nAChR antagonist alpha-bungarotoxin. The non-alpha 7 nAChR agonist 5-Iodo-A-85380 produces alpha-bungarotoxin-insensitive increases in intracellular Ca2+ (EC50 11.2 mu M). Using these selective agonists or KCl in conjunction with general and selective VOCC inhibitors, we demonstrate that the primary route of Ca2+ entry following either non-alpha 7 nAChR activation or KCl stimulation is via L-type VOCCs. In contrast, the alpha 7 nAChR-mediated response is unaffected by VOCC blockers but is inhibited by modulators of intracellular Ca2+ stores. These results indicate that alpha 7 and non-alpha 7 nAChRs are differentially coupled to Ca2+-induced Ca2+ release and VOCCs, respectively.

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DO - 10.1111/j.1471-4159.2006.04273.x

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SP - 1089

EP - 1096

JO - Journal of Neurochemistry

T2 - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

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ER -