Dicer1 is required for pigment cell and craniofacial development in zebrafish.

Andrea Weiner, Nadia Scampoli, Tomas Steeman, C. M. Dooley, Elisabeth Busch-Nentwich, Robert N. Kelsh, Nora Calcaterra

Research output: Contribution to journalArticle

Abstract

The multidomain RNase III endoribonuclease DICER is required for the generation of most functional microRNAs (miRNAs). Loss of Dicer affects developmental processes at different levels. Here, we characterized the zebrafish Dicer1 mutant, dicer1sa9205, which has a single point mutation induced by N-ethyl-N-nitrosourea mutagenesis. Heterozygous dicer1sa9205 developed normally, being phenotypically indistinguishable from wild-type siblings. Homozygous dicer1sa9205 mutants display smaller eyes, abnormal craniofacial development and aberrant pigmentation. Reduced numbers of both iridophores and melanocytes were observed in the head and ventral trunk of dicer1sa9205 homozygotes; the effect on melanocytes was stronger and detectable earlier in development. The expression of microphthalmia-associated transcription factor a (mitfa), the master gene for melanocytes differentiation, was enhanced in dicer1-depleted fish. Similarly, the expression of SRY-box containing gene 10 (sox10), required for mitfa activation, was higher in mutants than in wild types. In silico and in vivo analyses of either sox10 or mitfa 3’UTRs revealed conserved potential miRNA binding sites likely involved in the post-transcriptional regulation of both genes. Based on these findings, we propose that dicer1 participates in the gene regulatory network governing zebrafish melanocyte differentiation by controlling the expression of mitfa and sox10.
Original languageEnglish
Pages (from-to)472-485
Number of pages14
JournalBBA - Gene Regulatory Mechanisms
Volume1862
Issue number4
Early online date3 Mar 2019
DOIs
Publication statusPublished - 30 Apr 2019

Keywords

  • Neural crest
  • Zebrafish
  • miRNAs
  • sox10

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Weiner, A., Scampoli, N., Steeman, T., Dooley, C. M., Busch-Nentwich, E., Kelsh, R. N., & Calcaterra, N. (2019). Dicer1 is required for pigment cell and craniofacial development in zebrafish. BBA - Gene Regulatory Mechanisms, 1862(4), 472-485. https://doi.org/10.1016/j.bbagrm.2019.02.005

Dicer1 is required for pigment cell and craniofacial development in zebrafish. / Weiner, Andrea; Scampoli, Nadia; Steeman, Tomas; Dooley, C. M.; Busch-Nentwich, Elisabeth; Kelsh, Robert N.; Calcaterra, Nora.

In: BBA - Gene Regulatory Mechanisms, Vol. 1862, No. 4, 30.04.2019, p. 472-485.

Research output: Contribution to journalArticle

Weiner, A, Scampoli, N, Steeman, T, Dooley, CM, Busch-Nentwich, E, Kelsh, RN & Calcaterra, N 2019, 'Dicer1 is required for pigment cell and craniofacial development in zebrafish.', BBA - Gene Regulatory Mechanisms, vol. 1862, no. 4, pp. 472-485. https://doi.org/10.1016/j.bbagrm.2019.02.005
Weiner, Andrea ; Scampoli, Nadia ; Steeman, Tomas ; Dooley, C. M. ; Busch-Nentwich, Elisabeth ; Kelsh, Robert N. ; Calcaterra, Nora. / Dicer1 is required for pigment cell and craniofacial development in zebrafish. In: BBA - Gene Regulatory Mechanisms. 2019 ; Vol. 1862, No. 4. pp. 472-485.
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