Development of two novel automated fluoropolymer microfluidic platforms for high sensitivity multiplex diagnostic immunoassays

Nuno Reis, Filipa Pereira, Ana Barbosa, Ana Castanheira, Alexander Edwards

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our research team recently pioneered the development of ultra-sensitive sandwich ELISA in a novel melt-extruded microfluidic material called Microcapillary Film (MCF) [1-4]. Quantitative biomarker assays can be performed on whole blood in fluoropolymer MCF strips without the need of sample preparation [2], with simple optical detection with low-cost optoelectronic components including smartphones [3] that deliver limit-of-detection comparable to state-of-the-art clinical equipment [4]. To date, these assays were performed manually, and no automation of assays in MCF strips has yet been reported. Here, we report for the first time prototypes of two fully automated devices for performing ELISA in fluoropolymer MCF, using integrated low cost optoelectronic components for affordable signal quantitation.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages641-642
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 1 Jan 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16

    Fingerprint

Keywords

  • Automated microfluidics
  • Clinical diagnostics
  • Microcapillary Film
  • Microfluidic immunoassay

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Reis, N., Pereira, F., Barbosa, A., Castanheira, A., & Edwards, A. (2016). Development of two novel automated fluoropolymer microfluidic platforms for high sensitivity multiplex diagnostic immunoassays. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 641-642). Chemical and Biological Microsystems Society.