Abstract
| Language | English |
|---|---|
| Pages | 30-35 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 123 |
| Early online date | 26 Sep 2018 |
| DOIs | |
| Status | Published - 1 Jan 2019 |
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Keywords
- Biosensor
- Malaria
- PfGDH
- Aptamer
- field effect transistors (FETs)
Cite this
Development of an aptamer-based field effect transistor biosensor for quantitative detection of Plasmodium falciparum glutamate dehydrogenase in serum samples. / Singh, Naveen; Thungon, Phurpa Dema; Estrela, Pedro; Goswami, Pranab.
In: Biosensors and Bioelectronics, Vol. 123, 01.01.2019, p. 30-35.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of an aptamer-based field effect transistor biosensor for quantitative detection of Plasmodium falciparum glutamate dehydrogenase in serum samples
AU - Singh, Naveen
AU - Thungon, Phurpa Dema
AU - Estrela, Pedro
AU - Goswami, Pranab
PY - 2019/1/1
Y1 - 2019/1/1
N2 - There has been a continuous strive to develop portable, stable, sensitive and low cost detection system for malaria to meet the demand of effective screening actions in developing countries where the disease is most endemic. Herein, we report an aptamer-based field effect transistor (aptaFET) biosensor, developed by using an extended gate field effect transistor with inter-digitated gold microelectrodes (IDμE) for the detection of the malaria biomarker Plasmodium falciparum glutamate dehydrogenase (PfGDH) in serum samples. A 90 mer long ssDNA aptamer (NG3) selective to PfGDH was used in the aptaFET to capture the target protein. The intrinsic surface net charge of the captured protein led to change in gate potential of the aptaFET device, which could be correlated to the concentration of the protein. This biosensor exhibited a sensitive response in broad dynamic range of 100 fM - 10 nM with limits of detection of 16.7 pM and 48.6 pM in spiked buffer and serum samples, respectively. The high selectivity of the biosensor for PfGDH was verified by testing relevant analogous human and parasitic proteins on the device. Overall, the results validated the application potential of the developed aptaFET for diagnosis of both symptomatic and asymptomatic malaria.
AB - There has been a continuous strive to develop portable, stable, sensitive and low cost detection system for malaria to meet the demand of effective screening actions in developing countries where the disease is most endemic. Herein, we report an aptamer-based field effect transistor (aptaFET) biosensor, developed by using an extended gate field effect transistor with inter-digitated gold microelectrodes (IDμE) for the detection of the malaria biomarker Plasmodium falciparum glutamate dehydrogenase (PfGDH) in serum samples. A 90 mer long ssDNA aptamer (NG3) selective to PfGDH was used in the aptaFET to capture the target protein. The intrinsic surface net charge of the captured protein led to change in gate potential of the aptaFET device, which could be correlated to the concentration of the protein. This biosensor exhibited a sensitive response in broad dynamic range of 100 fM - 10 nM with limits of detection of 16.7 pM and 48.6 pM in spiked buffer and serum samples, respectively. The high selectivity of the biosensor for PfGDH was verified by testing relevant analogous human and parasitic proteins on the device. Overall, the results validated the application potential of the developed aptaFET for diagnosis of both symptomatic and asymptomatic malaria.
KW - Biosensor
KW - Malaria
KW - PfGDH
KW - Aptamer
KW - field effect transistors (FETs)
U2 - 10.1016/j.bios.2018.09.085
DO - 10.1016/j.bios.2018.09.085
M3 - Article
VL - 123
SP - 30
EP - 35
JO - Biosensors and Bioelectronics
T2 - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -