Development of an aptamer-based field effect transistor biosensor for quantitative detection of Plasmodium falciparum glutamate dehydrogenase in serum samples

Naveen Singh, Phurpa Dema Thungon, Pedro Estrela, Pranab Goswami

Research output: Contribution to journalArticle

Abstract

There has been a continuous strive to develop portable, stable, sensitive and low cost detection system for malaria to meet the demand of effective screening actions in developing countries where the disease is most endemic. Herein, we report an aptamer-based field effect transistor (aptaFET) biosensor, developed by using an extended gate field effect transistor with inter-digitated gold microelectrodes (IDμE) for the detection of the malaria biomarker Plasmodium falciparum glutamate dehydrogenase (PfGDH) in serum samples. A 90 mer long ssDNA aptamer (NG3) selective to PfGDH was used in the aptaFET to capture the target protein. The intrinsic surface net charge of the captured protein led to change in gate potential of the aptaFET device, which could be correlated to the concentration of the protein. This biosensor exhibited a sensitive response in broad dynamic range of 100 fM - 10 nM with limits of detection of 16.7 pM and 48.6 pM in spiked buffer and serum samples, respectively. The high selectivity of the biosensor for PfGDH was verified by testing relevant analogous human and parasitic proteins on the device. Overall, the results validated the application potential of the developed aptaFET for diagnosis of both symptomatic and asymptomatic malaria.
LanguageEnglish
Pages30-35
Number of pages6
JournalBiosensors and Bioelectronics
Volume123
Early online date26 Sep 2018
DOIs
StatusPublished - 1 Jan 2019

Fingerprint

Glutamate Dehydrogenase
Biosensing Techniques
Plasmodium falciparum
Field effect transistors
Biosensors
Proteins
Serum
Malaria
Gates (transistor)
Equipment and Supplies
Falciparum Malaria
Microelectrodes
Biomarkers
Developing countries
Gold
Developing Countries
Limit of Detection
Buffers
Screening
Costs and Cost Analysis

Keywords

  • Biosensor
  • Malaria
  • PfGDH
  • Aptamer
  • field effect transistors (FETs)

Cite this

Development of an aptamer-based field effect transistor biosensor for quantitative detection of Plasmodium falciparum glutamate dehydrogenase in serum samples. / Singh, Naveen; Thungon, Phurpa Dema; Estrela, Pedro; Goswami, Pranab.

In: Biosensors and Bioelectronics, Vol. 123, 01.01.2019, p. 30-35.

Research output: Contribution to journalArticle

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