Development of a synthetic medium for continuous anaerobic growth and ethanol production with a lactate dehydrogenase mutant of Bacillus stearothermophilus

R. San Martin, D. Bushell, D. J. Leak, B. S. Hartley

Research output: Contribution to journalArticle

Abstract

A synthetic medium was developed by the pulse and medium-shift technique for the continuous cultivation of Bacillus stearothermophilus strain LLD-15 (NCIMB 12428) under anaerobic conditions. This mutant strain lacks L-lactate dehydrogenase activity, and is a promising candidate for the production of ethanol from pentoses and hexoses, using a high-temperature two-stage process. The final medium contained four amino acids and five vitamins, and growth characteristics in this medium compared well with those in complex medium containing yeast extract and tryptone. At 70°C, the medium was capable of supporting good anaerobic and aerobic growth at 10 g input sucrose I-1. High ethanol production indicated that pyruvate metabolism probably occurred via the combined activity of the pyruvate-formate-lyase pathway and pyruvate dehydrogenase.

Original languageEnglish
Pages (from-to)987-996
Number of pages10
JournalJournal of General Microbiology
Volume138
Issue number5
Publication statusPublished - 15 Jun 1992

ASJC Scopus subject areas

  • Microbiology

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